Human IGFBP7/Igfbp Rp1 PicoKine® Quick ELISA Kit

Human IGFBP7

Product Specifications

Background

Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1401 1416 1369 1426 1403 44.90 3.2% Sample 2 2986 3531 3163 3154 3209 144.38 4.5% Sample 3 17423 15436 16428 16654 16485 560.5 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1401 1416 1369 1426 1403 44.90 3.2% Sample 1 1401 1416 1369 1426 1403 44.90 3.2% Sample 2 2986 3531 3163 3154 3209 144.38 4.5% Sample 2 2986 3531 3163 3154 3209 144.38 4.5% Sample 3 17423 15436 16428 16654 16485 560.5 3.4% Sample 3 17423 15436 16428 16654 16485 560.5 3.4%

Gene Name

IGFBP7

Gene ID

3490

UniProt

Q16270

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D30-L282

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1401 2986 17423 1521 3364 17532 Standard deviation 60.24 134.37 731.77 88.22 161.47 806.47 CV (%) 4.3% 4.5% 4.2% 5.8% 4.8% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1401 2986 17423 1521 3364 17532 Standard deviation 60.24 134.37 731.77 88.22 161.47 806.47 CV (%) 4.3% 4.5% 4.2% 5.8% 4.8% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1401 2986 17423 1521 3364 17532 Mean (pg/ml) 1401 2986 17423 1521 3364 17532 Standard deviation 60.24 134.37 731.77 88.22 161.47 806.47 Standard deviation 60.24 134.37 731.77 88.22 161.47 806.47 CV (%) 4.3% 4.5% 4.2% 5.8% 4.8% 4.6% CV (%) 4.3% 4.5% 4.2% 5.8% 4.8% 4.6%

Sensitivity

<300 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human IGFBP7

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 O.D. 0.029 0.092 0.143 0.237 0.443 0.848 1.570 2.243 Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 O.D. 0.029 0.092 0.143 0.237 0.443 0.848 1.570 2.243 Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 O.D. 0.029 0.092 0.143 0.237 0.443 0.848 1.570 2.243 O.D. 0.029 0.092 0.143 0.237 0.443 0.848 1.570 2.243

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.