Human CXCL4/PF4 PicoKine® Quick ELISA Kit

Human CXCL4/PF4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CXCL4/PF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4) . The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 366 303 355 314 335 23.08 6.9% Sample 2 1651 1700 1818 1664 1708 63.21 3.7% Sample 3 4823 4798 4635 4316 4643 190.36 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 366 303 355 314 335 23.08 6.9% Sample 1 366 303 355 314 335 23.08 6.9% Sample 2 1651 1700 1818 1664 1708 63.21 3.7% Sample 2 1651 1700 1818 1664 1708 63.21 3.7% Sample 3 4823 4798 4635 4316 4643 190.36 4.1% Sample 3 4823 4798 4635 4316 4643 190.36 4.1%

Gene Name

PF4

Gene ID

5196

UniProt

P02776

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E32-S101

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 366 1651 4823 342 1688 5103 Standard deviation 24.16 80.9 255.62 24.28 97.9 326.59 CV (%) 6.6% 4.9% 5.3% 7.1% 5.8% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 366 1651 4823 342 1688 5103 Standard deviation 24.16 80.9 255.62 24.28 97.9 326.59 CV (%) 6.6% 4.9% 5.3% 7.1% 5.8% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 366 1651 4823 342 1688 5103 Mean (pg/ml) 366 1651 4823 342 1688 5103 Standard deviation 24.16 80.9 255.62 24.28 97.9 326.59 Standard deviation 24.16 80.9 255.62 24.28 97.9 326.59 CV (%) 6.6% 4.9% 5.3% 7.1% 5.8% 6.4% CV (%) 6.6% 4.9% 5.3% 7.1% 5.8% 6.4%

Sensitivity

<15 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human PF4

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.017 0.149 0.244 0.438 0.875 1.606 2.272 2.492 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.017 0.149 0.244 0.438 0.875 1.606 2.272 2.492 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.017 0.149 0.244 0.438 0.875 1.606 2.272 2.492 O.D. 0.017 0.149 0.244 0.438 0.875 1.606 2.272 2.492

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.