Mouse CXCL4/PF4 PicoKine® Quick ELISA Kit

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4) . The PF4 gene was localized on map to 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 33 32 34 33 0.83 2.5% Sample 2 241 265 259 244 252 9.08 3.6% Sample 3 440 440 458 462 450 18.45 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 33 32 34 33 0.83 2.5% Sample 1 34 33 32 34 33 0.83 2.5% Sample 2 241 265 259 244 252 9.08 3.6% Sample 2 241 265 259 244 252 9.08 3.6% Sample 3 440 440 458 462 450 18.45 4.1% Sample 3 440 440 458 462 450 18.45 4.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 241 440 30 261 456 Standard deviation 1.73 11.57 28.16 1.92 16.18 31.46 CV (%) 5.1% 4.8% 6.4% 6.4% 6.2% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 241 440 30 261 456 Standard deviation 1.73 11.57 28.16 1.92 16.18 31.46 CV (%) 5.1% 4.8% 6.4% 6.4% 6.2% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 241 440 30 261 456 Mean (pg/ml) 34 241 440 30 261 456 Standard deviation 1.73 11.57 28.16 1.92 16.18 31.46 Standard deviation 1.73 11.57 28.16 1.92 16.18 31.46 CV (%) 5.1% 4.8% 6.4% 6.4% 6.2% 6.9% CV (%) 5.1% 4.8% 6.4% 6.4% 6.2% 6.9%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.050 0.148 0.224 0.379 0.685 1.249 1.544 2.098 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.050 0.148 0.224 0.379 0.685 1.249 1.544 2.098 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.050 0.148 0.224 0.379 0.685 1.249 1.544 2.098 O.D. 0.050 0.148 0.224 0.379 0.685 1.249 1.544 2.098

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.