Rat CXCL2/MIP-2 PicoKine® Quick ELISA Kit

Rat CXCL2/MIP-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat CXCL2/MIP-2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Macrophage Inflammatory Protein 2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 forms homotetramers. MIP2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. MIP2 is chemotactic for polymorphonuclear leukocytes.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 30 29 35 33 1.66 5.1% Sample 2 225 212 196 180 203 9.96 4.9% Sample 3 364 399 412 387 391 11.32 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 30 29 35 33 1.66 5.1% Sample 1 36 30 29 35 33 1.66 5.1% Sample 2 225 212 196 180 203 9.96 4.9% Sample 2 225 212 196 180 203 9.96 4.9% Sample 3 364 399 412 387 391 11.32 2.9% Sample 3 364 399 412 387 391 11.32 2.9%

Gene Name

Cxcl2

Gene ID

114105

UniProt

P30348

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S32-N100

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 225 364 40 196 377 Standard deviation 1.58 10.58 14.2 2.2 10 21.11 CV (%) 4.4% 4.7% 3.9 5.5% 5.1% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 225 364 40 196 377 Standard deviation 1.58 10.58 14.2 2.2 10 21.11 CV (%) 4.4% 4.7% 3.9 5.5% 5.1% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 225 364 40 196 377 Mean (pg/ml) 36 225 364 40 196 377 Standard deviation 1.58 10.58 14.2 2.2 10 21.11 Standard deviation 1.58 10.58 14.2 2.2 10 21.11 CV (%) 4.4% 4.7% 3.9 5.5% 5.1% 5.6% CV (%) 4.4% 4.7% 3.9 5.5% 5.1% 5.6%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Rat Cxcl2

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.060 0.233 0.380 0.672 1.188 1.948 2.349 2.470 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.060 0.233 0.380 0.672 1.188 1.948 2.349 2.470 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.060 0.233 0.380 0.672 1.188 1.948 2.349 2.470 O.D. 0.060 0.233 0.380 0.672 1.188 1.948 2.349 2.470

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.