Rat CXCL2/MIP-2 PicoKine® Quick ELISA Kit

Macrophage Inflammatory Protein 2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 forms homotetramers. MIP2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. MIP2 is chemotactic for polymorphonuclear leukocytes.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 30 29 35 33 1.66 5.1% Sample 2 225 212 196 180 203 9.96 4.9% Sample 3 364 399 412 387 391 11.32 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 30 29 35 33 1.66 5.1% Sample 1 36 30 29 35 33 1.66 5.1% Sample 2 225 212 196 180 203 9.96 4.9% Sample 2 225 212 196 180 203 9.96 4.9% Sample 3 364 399 412 387 391 11.32 2.9% Sample 3 364 399 412 387 391 11.32 2.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 225 364 40 196 377 Standard deviation 1.58 10.58 14.2 2.2 10 21.11 CV (%) 4.4% 4.7% 3.9 5.5% 5.1% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 225 364 40 196 377 Standard deviation 1.58 10.58 14.2 2.2 10 21.11 CV (%) 4.4% 4.7% 3.9 5.5% 5.1% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 225 364 40 196 377 Mean (pg/ml) 36 225 364 40 196 377 Standard deviation 1.58 10.58 14.2 2.2 10 21.11 Standard deviation 1.58 10.58 14.2 2.2 10 21.11 CV (%) 4.4% 4.7% 3.9 5.5% 5.1% 5.6% CV (%) 4.4% 4.7% 3.9 5.5% 5.1% 5.6%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.060 0.233 0.380 0.672 1.188 1.948 2.349 2.470 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.060 0.233 0.380 0.672 1.188 1.948 2.349 2.470 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.060 0.233 0.380 0.672 1.188 1.948 2.349 2.470 O.D. 0.060 0.233 0.380 0.672 1.188 1.948 2.349 2.470

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.