Human CXCL9/Mig PicoKine® Quick ELISA Kit

Human CXCL9/Mig PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CXCL9/Mig in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG) . CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4.1, 2 CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3.3 The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 61 55 56 57 2.52 4.4% Sample 2 288 284 296 291 290 10.43 3.6% Sample 3 840 853 871 901 866 44.18 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 61 55 56 57 2.52 4.4% Sample 1 57 61 55 56 57 2.52 4.4% Sample 2 288 284 296 291 290 10.43 3.6% Sample 2 288 284 296 291 290 10.43 3.6% Sample 3 840 853 871 901 866 44.18 5.1% Sample 3 840 853 871 901 866 44.18 5.1%

Gene Name

CXCL9

Gene ID

4283

UniProt

Q07325

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T23-T125

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 288 840 56 314 797 Standard deviation 4.79 21.6 51.24 4.03 23.24 51.81 CV (%) 8.4% 7.5% 6.1% 7.2% 7.4% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 288 840 56 314 797 Standard deviation 4.79 21.6 51.24 4.03 23.24 51.81 CV (%) 8.4% 7.5% 6.1% 7.2% 7.4% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 57 288 840 56 314 797 Mean (pg/ml) 57 288 840 56 314 797 Standard deviation 4.79 21.6 51.24 4.03 23.24 51.81 Standard deviation 4.79 21.6 51.24 4.03 23.24 51.81 CV (%) 8.4% 7.5% 6.1% 7.2% 7.4% 6.5% CV (%) 8.4% 7.5% 6.1% 7.2% 7.4% 6.5%

Sensitivity

<2 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CXCL9

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.037 0.167 0.262 0.441 0.777 1.207 1.658 2.021 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.037 0.167 0.262 0.441 0.777 1.207 1.658 2.021 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.037 0.167 0.262 0.441 0.777 1.207 1.658 2.021 O.D. 0.037 0.167 0.262 0.441 0.777 1.207 1.658 2.021

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.