Human CD320/8D6A PicoKine® Quick ELISA Kit

Human CD320/8D6A PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CD320/8D6A in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD320 (cluster of differentiation 320), also known as 8D6A or TCBLR, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and the expression of CD320 enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the CD320 signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. CD320 is also a specific receptor for uptake of TCN2-bound cobalamin.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 301 245 283 273 9.29 3.4% Sample 2 941 882 1041 996 965 53.08 5.5% Sample 3 2154 2260 2312 2041 2192 105.2 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 301 245 283 273 9.29 3.4% Sample 1 264 301 245 283 273 9.29 3.4% Sample 2 941 882 1041 996 965 53.08 5.5% Sample 2 941 882 1041 996 965 53.08 5.5% Sample 3 2154 2260 2312 2041 2192 105.2 4.8% Sample 3 2154 2260 2312 2041 2192 105.2 4.8%

Gene Name

CD320

Gene ID

51293

UniProt

Q9NPF0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S36-Y229

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 941 2154 301 1004 2278 Standard deviation 13.46 53.64 127.09 15.65 69.28 145.79 CV (%) 5.1% 5.7% 5.9% 5.2% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 941 2154 301 1004 2278 Standard deviation 13.46 53.64 127.09 15.65 69.28 145.79 CV (%) 5.1% 5.7% 5.9% 5.2% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 264 941 2154 301 1004 2278 Mean (pg/ml) 264 941 2154 301 1004 2278 Standard deviation 13.46 53.64 127.09 15.65 69.28 145.79 Standard deviation 13.46 53.64 127.09 15.65 69.28 145.79 CV (%) 5.1% 5.7% 5.9% 5.2% 6.9% 6.4% CV (%) 5.1% 5.7% 5.9% 5.2% 6.9% 6.4%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CD320

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.067 0.179 0.254 0.444 0.704 1.245 1.739 2.243 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.067 0.179 0.254 0.444 0.704 1.245 1.739 2.243 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.067 0.179 0.254 0.444 0.704 1.245 1.739 2.243 O.D. 0.067 0.179 0.254 0.444 0.704 1.245 1.739 2.243

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.