Human CD206/CLEC13D PicoKine® Quick ELISA Kit

Human CD206/CLEC13D PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CD206/CLEC13D in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages and immature dendritic cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205) . This gene is mapped to 10p12.33. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1214 1347 1449 1542 1388 68.01 4.9% Sample 2 5652 6145 5784 5912 5873 340.65 5.8% Sample 3 25412 26874 29748 25944 26995 1754.64 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1214 1347 1449 1542 1388 68.01 4.9% Sample 1 1214 1347 1449 1542 1388 68.01 4.9% Sample 2 5652 6145 5784 5912 5873 340.65 5.8% Sample 2 5652 6145 5784 5912 5873 340.65 5.8% Sample 3 25412 26874 29748 25944 26995 1754.64 6.5% Sample 3 25412 26874 29748 25944 26995 1754.64 6.5%

Gene Name

MRC1

Gene ID

4360

UniProt

P22897

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L19-K1383

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1214 5652 25412 1333 6144 26014 Standard deviation 75.27 356.08 1296.01 85.31 423.94 1794.97 CV (%) 6.2% 6.3% 5.1% 6.4% 6.9% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1214 5652 25412 1333 6144 26014 Standard deviation 75.27 356.08 1296.01 85.31 423.94 1794.97 CV (%) 6.2% 6.3% 5.1% 6.4% 6.9% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1214 5652 25412 1333 6144 26014 Mean (pg/ml) 1214 5652 25412 1333 6144 26014 Standard deviation 75.27 356.08 1296.01 85.31 423.94 1794.97 Standard deviation 75.27 356.08 1296.01 85.31 423.94 1794.97 CV (%) 6.2% 6.3% 5.1% 6.4% 6.9% 6.9% CV (%) 6.2% 6.3% 5.1% 6.4% 6.9% 6.9%

Sensitivity

<50 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human MRC1

Anticoagulant

Heparin, EDTA or citrate

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.034 0.179 0.285 0.502 0.883 1.461 2.019 2.326 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.034 0.179 0.285 0.502 0.883 1.461 2.019 2.326 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.034 0.179 0.285 0.502 0.883 1.461 2.019 2.326 O.D. 0.034 0.179 0.285 0.502 0.883 1.461 2.019 2.326

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.