Human CD164 PicoKine® Quick ELISA Kit

Human CD164 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CD164 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Sialomucin core protein 24 also known as endolyn or CD164, is a protein that in humans is encoded by the CD164 gene. CD164 functions as a cell adhesion molecule. This gene is mapped to 6q21. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 135 144 180 141 6.35 4.5% Sample 2 366 370 285 294 329 16.77 5.1% Sample 3 847 904 1006 1167 981 22.56 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 135 144 180 141 6.35 4.5% Sample 1 105 135 144 180 141 6.35 4.5% Sample 2 366 370 285 294 329 16.77 5.1% Sample 2 366 370 285 294 329 16.77 5.1% Sample 3 847 904 1006 1167 981 22.56 2.3% Sample 3 847 904 1006 1167 981 22.56 2.3%

Gene Name

CD164

Gene ID

8763

UniProt

Q04900

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D24-D162

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

93.7 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 366 847 124 456 964 Standard deviation 5.67 15.01 46.59 7.56 28.73 67.48 CV (%) 5.4% 4.1% 5.5% 6.1% 6.3% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 366 847 124 456 964 Standard deviation 5.67 15.01 46.59 7.56 28.73 67.48 CV (%) 5.4% 4.1% 5.5% 6.1% 6.3% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 105 366 847 124 456 964 Mean (pg/ml) 105 366 847 124 456 964 Standard deviation 5.67 15.01 46.59 7.56 28.73 67.48 Standard deviation 5.67 15.01 46.59 7.56 28.73 67.48 CV (%) 5.4% 4.1% 5.5% 6.1% 6.3% 7.0% CV (%) 5.4% 4.1% 5.5% 6.1% 6.3% 7.0%

Sensitivity

<50 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CD164

Anticoagulant

Heparin, EDTA or citrate

Sample Data

Concentration (pg/ml) 0 93.7 187.5 375 750 1,500 3,000 6,000 O.D. 0.079 0.180 0.266 0.410 0.667 1.083 1.610 2.099 Concentration (pg/ml) 0 93.7 187.5 375 750 1,500 3,000 6,000 O.D. 0.079 0.180 0.266 0.410 0.667 1.083 1.610 2.099 Concentration (pg/ml) 0 93.7 187.5 375 750 1,500 3,000 6,000 Concentration (pg/ml) 0 93.7 187.5 375 750 1,500 3,000 6,000 O.D. 0.079 0.180 0.266 0.410 0.667 1.083 1.610 2.099 O.D. 0.079 0.180 0.266 0.410 0.667 1.083 1.610 2.099

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.