Human CCL1 PicoKine® Quick ELISA Kit

Human CCL1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CCL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 30 30 30 0.99 3.3% Sample 2 198 179 183 181 185 8.69 4.7% Sample 3 434 460 435 417 436 19.62 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 30 30 30 0.99 3.3% Sample 1 32 30 30 30 30 0.99 3.3% Sample 2 198 179 183 181 185 8.69 4.7% Sample 2 198 179 183 181 185 8.69 4.7% Sample 3 434 460 435 417 436 19.62 4.5% Sample 3 434 460 435 417 436 19.62 4.5%

Gene Name

CCL1

Gene ID

6346

UniProt

P22362

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K24-K96

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 198 434 33 194 408 Standard deviation 2.30 12.47 32.55 2.67 14.55 35.90 CV (%) 7.2% 6.3% 7.5% 8.1% 7.5% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 198 434 33 194 408 Standard deviation 2.30 12.47 32.55 2.67 14.55 35.90 CV (%) 7.2% 6.3% 7.5% 8.1% 7.5% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 198 434 33 194 408 Mean (pg/ml) 32 198 434 33 194 408 Standard deviation 2.30 12.47 32.55 2.67 14.55 35.90 Standard deviation 2.30 12.47 32.55 2.67 14.55 35.90 CV (%) 7.2% 6.3% 7.5% 8.1% 7.5% 8.8% CV (%) 7.2% 6.3% 7.5% 8.1% 7.5% 8.8%

Sensitivity

<15 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CCL1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.132 0.222 0.410 0.739 1.333 2.024 2.158 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.132 0.222 0.410 0.739 1.333 2.024 2.158 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.132 0.222 0.410 0.739 1.333 2.024 2.158 O.D. 0.009 0.132 0.222 0.410 0.739 1.333 2.024 2.158

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.