Mouse CCL7/MCP3 PicoKine® Quick ELISA Kit

Mouse CCL7/MCP3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse CCL7/MCP3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3) . It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 36 34 34 34 0.78 2.3% Sample 2 196 220 207 218 210 9.87 4.7% Sample 3 461 443 415 486 451 24.80 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 36 34 34 34 0.78 2.3% Sample 1 35 36 34 34 34 0.78 2.3% Sample 2 196 220 207 218 210 9.87 4.7% Sample 2 196 220 207 218 210 9.87 4.7% Sample 3 461 443 415 486 451 24.80 5.5% Sample 3 461 443 415 486 451 24.80 5.5%

Gene Name

Ccl7

Gene ID

20306

UniProt

Q03366

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-P97

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 196 461 33 178 494 Standard deviation 1.50 12.74 28.12 2.90 13.35 43.47 CV (%) 4.3% 6.5% 6.1% 8.8% 7.5% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 196 461 33 178 494 Standard deviation 1.50 12.74 28.12 2.90 13.35 43.47 CV (%) 4.3% 6.5% 6.1% 8.8% 7.5% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 196 461 33 178 494 Mean (pg/ml) 35 196 461 33 178 494 Standard deviation 1.50 12.74 28.12 2.90 13.35 43.47 Standard deviation 1.50 12.74 28.12 2.90 13.35 43.47 CV (%) 4.3% 6.5% 6.1% 8.8% 7.5% 8.8% CV (%) 4.3% 6.5% 6.1% 8.8% 7.5% 8.8%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Ccl7

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.025 0.171 0.307 0.552 0.946 1.611 2.060 2.226 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.025 0.171 0.307 0.552 0.946 1.611 2.060 2.226 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.025 0.171 0.307 0.552 0.946 1.611 2.060 2.226 O.D. 0.025 0.171 0.307 0.552 0.946 1.611 2.060 2.226

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.