Human APLP1 PicoKine® Quick ELISA Kit

Human APLP1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human APLP1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

This gene encodes a member of the highly conserved amyloid precursor protein gene family. It is mapped to 19q13.12. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 211 253 236 183 13.90 7.6% Sample 2 972 1440 1371 1396 1294 54.34 4.2% Sample 3 3395 4159 3479 3643 3669 194.45 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 211 253 236 183 13.90 7.6% Sample 1 270 211 253 236 183 13.90 7.6% Sample 2 972 1440 1371 1396 1294 54.34 4.2% Sample 2 972 1440 1371 1396 1294 54.34 4.2% Sample 3 3395 4159 3479 3643 3669 194.45 5.3% Sample 3 3395 4159 3479 3643 3669 194.45 5.3%

Gene Name

APLP1

Gene ID

333

UniProt

P51693

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S39-E580

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 972 3395 311 1030 4066 Standard deviation 11.34 43.47 166.35 15.86 66.95 300.88 CV (%) 4.2% 4.5% 4.9% 5.1% 6.5% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 972 3395 311 1030 4066 Standard deviation 11.34 43.47 166.35 15.86 66.95 300.88 CV (%) 4.2% 4.5% 4.9% 5.1% 6.5% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 270 972 3395 311 1030 4066 Mean (pg/ml) 270 972 3395 311 1030 4066 Standard deviation 11.34 43.47 166.35 15.86 66.95 300.88 Standard deviation 11.34 43.47 166.35 15.86 66.95 300.88 CV (%) 4.2% 4.5% 4.9% 5.1% 6.5% 7.4% CV (%) 4.2% 4.5% 4.9% 5.1% 6.5% 7.4%

Sensitivity

<50 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human APLP1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.