Human APLP1 PicoKine® Quick ELISA Kit

This gene encodes a member of the highly conserved amyloid precursor protein gene family. It is mapped to 19q13.12. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 211 253 236 183 13.90 7.6% Sample 2 972 1440 1371 1396 1294 54.34 4.2% Sample 3 3395 4159 3479 3643 3669 194.45 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 211 253 236 183 13.90 7.6% Sample 1 270 211 253 236 183 13.90 7.6% Sample 2 972 1440 1371 1396 1294 54.34 4.2% Sample 2 972 1440 1371 1396 1294 54.34 4.2% Sample 3 3395 4159 3479 3643 3669 194.45 5.3% Sample 3 3395 4159 3479 3643 3669 194.45 5.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 972 3395 311 1030 4066 Standard deviation 11.34 43.47 166.35 15.86 66.95 300.88 CV (%) 4.2% 4.5% 4.9% 5.1% 6.5% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 972 3395 311 1030 4066 Standard deviation 11.34 43.47 166.35 15.86 66.95 300.88 CV (%) 4.2% 4.5% 4.9% 5.1% 6.5% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 270 972 3395 311 1030 4066 Mean (pg/ml) 270 972 3395 311 1030 4066 Standard deviation 11.34 43.47 166.35 15.86 66.95 300.88 Standard deviation 11.34 43.47 166.35 15.86 66.95 300.88 CV (%) 4.2% 4.5% 4.9% 5.1% 6.5% 7.4% CV (%) 4.2% 4.5% 4.9% 5.1% 6.5% 7.4%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.