Human Cathepsin S PicoKine® Quick ELISA Kit

Human Cathepsin S PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human Cathepsin S in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cathepsin S, also known as CTSS, is a protein that in humans is encoded by the CTSS gene. A member of the peptidase C1 family, CTSS gene is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. By fluorescence in situ hybridization (FISH), this gene is mapped to 1q21.3. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 179 186 175 193 183 6.58 3.6% Sample 2 406 440 371 396 403 24.98 6.2% Sample 3 501 531 479 463 493 25.63 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 179 186 175 193 183 6.58 3.6% Sample 1 179 186 175 193 183 6.58 3.6% Sample 2 406 440 371 396 403 24.98 6.2% Sample 2 406 440 371 396 403 24.98 6.2% Sample 3 501 531 479 463 493 25.63 5.2% Sample 3 501 531 479 463 493 25.63 5.2%

Gene Name

CTSS

Gene ID

1520

UniProt

P25774

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q17-I331 & S109-I331

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 179 406 1977 190 417 1912 Standard deviation 9.30 19.48 136.41 11.59 27.10 149.13 CV (%) 5.2% 4.8% 6.9% 6.1% 6.5% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 179 406 1977 190 417 1912 Standard deviation 9.30 19.48 136.41 11.59 27.10 149.13 CV (%) 5.2% 4.8% 6.9% 6.1% 6.5% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 179 406 1977 190 417 1912 Mean (pg/ml) 179 406 1977 190 417 1912 Standard deviation 9.30 19.48 136.41 11.59 27.10 149.13 Standard deviation 9.30 19.48 136.41 11.59 27.10 149.13 CV (%) 5.2% 4.8% 6.9% 6.1% 6.5% 7.8% CV (%) 5.2% 4.8% 6.9% 6.1% 6.5% 7.8%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CTSS

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.051 0.192 0.296 0.512 0.884 1.530 2.209 2.316 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.051 0.192 0.296 0.512 0.884 1.530 2.209 2.316 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.051 0.192 0.296 0.512 0.884 1.530 2.209 2.316 O.D. 0.051 0.192 0.296 0.512 0.884 1.530 2.209 2.316

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.