Human APOE PicoKine® Quick ELISA Kit

Human APOE PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human APOE in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, milk and urine. Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-absorbance lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low absorbance lipoprotein receptor gene family. This protein is involved in Alzheimer’s disease and cardiovascular disease.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 7336 7271 6566 6791 6991 265.65 3.8% Sample 2 30862 28096 32231 27122 29577 1449.27 4.9% Sample 3 101167 109927 102578 89024 100674 6241.78 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 7336 7271 6566 6791 6991 265.65 3.8% Sample 1 7336 7271 6566 6791 6991 265.65 3.8% Sample 2 30862 28096 32231 27122 29577 1449.27 4.9% Sample 2 30862 28096 32231 27122 29577 1449.27 4.9% Sample 3 101167 109927 102578 89024 100674 6241.78 6.2% Sample 3 101167 109927 102578 89024 100674 6241.78 6.2%

Gene Name

APOE

Gene ID

348

UniProt

P02649

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: K19-H317

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

200 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, milk and urine

Detection Range

3.12 ng/mL - 200 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 7336 30862 101167 7284 33941 97075 Standard deviation 454.83 2129.47 7587.52 590.00 2884.98 8542.60 CV (%) 6.2% 6.9% 7.5% 8.1% 8.5% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 7336 30862 101167 7284 33941 97075 Standard deviation 454.83 2129.47 7587.52 590.00 2884.98 8542.60 CV (%) 6.2% 6.9% 7.5% 8.1% 8.5% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 7336 30862 101167 7284 33941 97075 Mean (pg/ml) 7336 30862 101167 7284 33941 97075 Standard deviation 454.83 2129.47 7587.52 590.00 2884.98 8542.60 Standard deviation 454.83 2129.47 7587.52 590.00 2884.98 8542.60 CV (%) 6.2% 6.9% 7.5% 8.1% 8.5% 8.8% CV (%) 6.2% 6.9% 7.5% 8.1% 8.5% 8.8%

Sensitivity

<50 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human APOE

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.003 0.143 0.256 0.448 0.731 1.041 1.413 1.650 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.003 0.143 0.256 0.448 0.731 1.041 1.413 1.650 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.003 0.143 0.256 0.448 0.731 1.041 1.413 1.650 O.D. 0.003 0.143 0.256 0.448 0.731 1.041 1.413 1.650

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.