Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit

Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human EMMPRIN/CD147/BSG in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147 (cluster of differentiation 147) . It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition (Miyauchi et al., 1991; Kanekura et al., 1991) .This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 71 71 66 67 68 2.58 3.8% Sample 2 234 209 223 212 219 11.38 5.2% Sample 3 1067 994 957 1024 1010 45.45 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 71 71 66 67 68 2.58 3.8% Sample 1 71 71 66 67 68 2.58 3.8% Sample 2 234 209 223 212 219 11.38 5.2% Sample 2 234 209 223 212 219 11.38 5.2% Sample 3 1067 994 957 1024 1010 45.45 4.5% Sample 3 1067 994 957 1024 1010 45.45 4.5%

Gene Name

BSG

Gene ID

682

UniProt

P35613

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T25-H205

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 71 234 1067 72 212 977 Standard deviation 4.68 16.84 84.29 5.40 15.90 79.13 CV (%) 6.6% 7.2% 7.9% 7.5% 7.7% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 71 234 1067 72 212 977 Standard deviation 4.68 16.84 84.29 5.40 15.90 79.13 CV (%) 6.6% 7.2% 7.9% 7.5% 7.7% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 71 234 1067 72 212 977 Mean (pg/ml) 71 234 1067 72 212 977 Standard deviation 4.68 16.84 84.29 5.40 15.90 79.13 Standard deviation 4.68 16.84 84.29 5.40 15.90 79.13 CV (%) 6.6% 7.2% 7.9% 7.5% 7.7% 8.1% CV (%) 6.6% 7.2% 7.9% 7.5% 7.7% 8.1%

Sensitivity

<2 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human BSG

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.029 0.097 0.155 0.262 0.484 0.879 1.548 2.261 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.029 0.097 0.155 0.262 0.484 0.879 1.548 2.261 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.029 0.097 0.155 0.262 0.484 0.879 1.548 2.261 O.D. 0.029 0.097 0.155 0.262 0.484 0.879 1.548 2.261

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.