Human TNFRSF13C/BAFFR PicoKine® Quick ELISA Kit

Human TNFRSF13C/BAFFR PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human TNFRSF13C/BAFFR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 179 164 137 140 155 10.54 6.8% Sample 2 685 749 711 654 699 35.64 5.1% Sample 3 2752 3118 2754 2810 2858 157.19 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 179 164 137 140 155 10.54 6.8% Sample 1 179 164 137 140 155 10.54 6.8% Sample 2 685 749 711 654 699 35.64 5.1% Sample 2 685 749 711 654 699 35.64 5.1% Sample 3 2752 3118 2754 2810 2858 157.19 5.5% Sample 3 2752 3118 2754 2810 2858 157.19 5.5%

Gene Name

TNFRSF13C

Gene ID

115650

UniProt

Q96RJ3

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S7-A71

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 179 685 2752 158 702 2533 Standard deviation 13.06 51.37 217.40 13.43 61.07 210.23 CV (%) 7.3% 7.5% 7.9% 8.5% 8.7% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 179 685 2752 158 702 2533 Standard deviation 13.06 51.37 217.40 13.43 61.07 210.23 CV (%) 7.3% 7.5% 7.9% 8.5% 8.7% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 179 685 2752 158 702 2533 Mean (pg/ml) 179 685 2752 158 702 2533 Standard deviation 13.06 51.37 217.40 13.43 61.07 210.23 Standard deviation 13.06 51.37 217.40 13.43 61.07 210.23 CV (%) 7.3% 7.5% 7.9% 8.5% 8.7% 8.3% CV (%) 7.3% 7.5% 7.9% 8.5% 8.7% 8.3%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human TNFRSF13C

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.031 0.120 0.192 0.341 0.604 1.071 1.875 2.426 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.031 0.120 0.192 0.341 0.604 1.071 1.875 2.426 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.031 0.120 0.192 0.341 0.604 1.071 1.875 2.426 O.D. 0.031 0.120 0.192 0.341 0.604 1.071 1.875 2.426

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.