Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit

Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high absorbance lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 8188 7998 8004 7332 7880 330.96 4.2% Sample 2 19829 19750 20658 19659 19974 619.19 3.1% Sample 3 93784 86245 87457 88574 89015 4005.67 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 8188 7998 8004 7332 7880 330.96 4.2% Sample 1 8188 7998 8004 7332 7880 330.96 4.2% Sample 2 19829 19750 20658 19659 19974 619.19 3.1% Sample 2 19829 19750 20658 19659 19974 619.19 3.1% Sample 3 93784 86245 87457 88574 89015 4005.67 4.5% Sample 3 93784 86245 87457 88574 89015 4005.67 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 8188 19829 93784 8185 19184 85441 Standard deviation 368.46 1249.22 5533.25 532.02 1477.16 5382.78 CV (%) 4.5% 6.3% 5.9% 6.5% 7.7% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 8188 19829 93784 8185 19184 85441 Standard deviation 368.46 1249.22 5533.25 532.02 1477.16 5382.78 CV (%) 4.5% 6.3% 5.9% 6.5% 7.7% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 8188 19829 93784 8185 19184 85441 Mean (pg/ml) 8188 19829 93784 8185 19184 85441 Standard deviation 368.46 1249.22 5533.25 532.02 1477.16 5382.78 Standard deviation 368.46 1249.22 5533.25 532.02 1477.16 5382.78 CV (%) 4.5% 6.3% 5.9% 6.5% 7.7% 6.3% CV (%) 4.5% 6.3% 5.9% 6.5% 7.7% 6.3%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.021 0.157 0.271 0.483 0.814 1.311 1.870 2.285 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.021 0.157 0.271 0.483 0.814 1.311 1.870 2.285 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.021 0.157 0.271 0.483 0.814 1.311 1.870 2.285 O.D. 0.021 0.157 0.271 0.483 0.814 1.311 1.870 2.285

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.