Human CD56/NCAM-1 PicoKine® Quick ELISA Kit

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 321 343 315 299 319 16.58 5.2% Sample 2 1087 1191 1033 1021 1083 6.06 6.1% Sample 3 5574 5246 5975 5765 5640 253.80 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 321 343 315 299 319 16.58 5.2% Sample 1 321 343 315 299 319 16.58 5.2% Sample 2 1087 1191 1033 1021 1083 6.06 6.1% Sample 2 1087 1191 1033 1021 1083 6.06 6.1% Sample 3 5574 5246 5975 5765 5640 253.80 4.5% Sample 3 5574 5246 5975 5765 5640 253.80 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 321 1087 5574 316 1104 5561 Standard deviation 17.65 47.82 273.12 18.01 51.88 294.73 CV (%) 5.5% 4.4% 4.9% 5.7% 4.7% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 321 1087 5574 316 1104 5561 Standard deviation 17.65 47.82 273.12 18.01 51.88 294.73 CV (%) 5.5% 4.4% 4.9% 5.7% 4.7% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 321 1087 5574 316 1104 5561 Mean (pg/ml) 321 1087 5574 316 1104 5561 Standard deviation 17.65 47.82 273.12 18.01 51.88 294.73 Standard deviation 17.65 47.82 273.12 18.01 51.88 294.73 CV (%) 5.5% 4.4% 4.9% 5.7% 4.7% 5.3% CV (%) 5.5% 4.4% 4.9% 5.7% 4.7% 5.3%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.091 0.161 0.209 0.321 0.544 1.031 1.529 2.195 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.091 0.161 0.209 0.321 0.544 1.031 1.529 2.195 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.091 0.161 0.209 0.321 0.544 1.031 1.529 2.195 O.D. 0.091 0.161 0.209 0.321 0.544 1.031 1.529 2.195

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.