Human CD56/NCAM-1 PicoKine® Quick ELISA Kit

Human CD56/NCAM-1 PicoKine® Quick ELISA Kit PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CD56/NCAM-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 321 343 315 299 319 16.58 5.2% Sample 2 1087 1191 1033 1021 1083 6.06 6.1% Sample 3 5574 5246 5975 5765 5640 253.80 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 321 343 315 299 319 16.58 5.2% Sample 1 321 343 315 299 319 16.58 5.2% Sample 2 1087 1191 1033 1021 1083 6.06 6.1% Sample 2 1087 1191 1033 1021 1083 6.06 6.1% Sample 3 5574 5246 5975 5765 5640 253.80 4.5% Sample 3 5574 5246 5975 5765 5640 253.80 4.5%

Gene Name

NCAM1

Gene ID

4684

UniProt

P13591

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-G718

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 321 1087 5574 316 1104 5561 Standard deviation 17.65 47.82 273.12 18.01 51.88 294.73 CV (%) 5.5% 4.4% 4.9% 5.7% 4.7% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 321 1087 5574 316 1104 5561 Standard deviation 17.65 47.82 273.12 18.01 51.88 294.73 CV (%) 5.5% 4.4% 4.9% 5.7% 4.7% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 321 1087 5574 316 1104 5561 Mean (pg/ml) 321 1087 5574 316 1104 5561 Standard deviation 17.65 47.82 273.12 18.01 51.88 294.73 Standard deviation 17.65 47.82 273.12 18.01 51.88 294.73 CV (%) 5.5% 4.4% 4.9% 5.7% 4.7% 5.3% CV (%) 5.5% 4.4% 4.9% 5.7% 4.7% 5.3%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human NCAM1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.091 0.161 0.209 0.321 0.544 1.031 1.529 2.195 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.091 0.161 0.209 0.321 0.544 1.031 1.529 2.195 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.091 0.161 0.209 0.321 0.544 1.031 1.529 2.195 O.D. 0.091 0.161 0.209 0.321 0.544 1.031 1.529 2.195

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.