Human Annexin A1 PicoKine® Quick ELISA Kit

Human Annexin A1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human ANXA1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 100pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca (2+) -dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca (2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 291 294 288 290 6.39 2.2% Sample 2 1452 1426 1367 1348 1398 27.97 2% Sample 3 5263 4796 5102 4982 5036 125.89 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 291 294 288 290 6.39 2.2% Sample 1 288 291 294 288 290 6.39 2.2% Sample 2 1452 1426 1367 1348 1398 27.97 2% Sample 2 1452 1426 1367 1348 1398 27.97 2% Sample 3 5263 4796 5102 4982 5036 125.89 2.5% Sample 3 5263 4796 5102 4982 5036 125.89 2.5%

Gene Name

CST3

Gene ID

301

UniProt

P04083

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A2-N346

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1452 5263 301 1563 5364 Standard deviation 12.67 59.53 268.41 17.16 100.03 413.03 CV (%) 4.4% 4.1% 5.1% 5.7% 6.4% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1452 5263 301 1563 5364 Standard deviation 12.67 59.53 268.41 17.16 100.03 413.03 CV (%) 4.4% 4.1% 5.1% 5.7% 6.4% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 288 1452 5263 301 1563 5364 Mean (pg/ml) 288 1452 5263 301 1563 5364 Standard deviation 12.67 59.53 268.41 17.16 100.03 413.03 Standard deviation 12.67 59.53 268.41 17.16 100.03 413.03 CV (%) 4.4% 4.1% 5.1% 5.7% 6.4% 7.7% CV (%) 4.4% 4.1% 5.1% 5.7% 6.4% 7.7%

Sensitivity

<100 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human ANXA1

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Annexin A1

Protein Name

Annexin A1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.128 0.173 0.274 0.458 0.833 1.436 2.197 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.128 0.173 0.274 0.458 0.833 1.436 2.197 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.128 0.173 0.274 0.458 0.833 1.436 2.197 O.D. 0.056 0.128 0.173 0.274 0.458 0.833 1.436 2.197

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.