Human Annexin A1 PicoKine® Quick ELISA Kit

ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca (2+) -dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca (2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 291 294 288 290 6.39 2.2% Sample 2 1452 1426 1367 1348 1398 27.97 2% Sample 3 5263 4796 5102 4982 5036 125.89 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 291 294 288 290 6.39 2.2% Sample 1 288 291 294 288 290 6.39 2.2% Sample 2 1452 1426 1367 1348 1398 27.97 2% Sample 2 1452 1426 1367 1348 1398 27.97 2% Sample 3 5263 4796 5102 4982 5036 125.89 2.5% Sample 3 5263 4796 5102 4982 5036 125.89 2.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1452 5263 301 1563 5364 Standard deviation 12.67 59.53 268.41 17.16 100.03 413.03 CV (%) 4.4% 4.1% 5.1% 5.7% 6.4% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1452 5263 301 1563 5364 Standard deviation 12.67 59.53 268.41 17.16 100.03 413.03 CV (%) 4.4% 4.1% 5.1% 5.7% 6.4% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 288 1452 5263 301 1563 5364 Mean (pg/ml) 288 1452 5263 301 1563 5364 Standard deviation 12.67 59.53 268.41 17.16 100.03 413.03 Standard deviation 12.67 59.53 268.41 17.16 100.03 413.03 CV (%) 4.4% 4.1% 5.1% 5.7% 6.4% 7.7% CV (%) 4.4% 4.1% 5.1% 5.7% 6.4% 7.7%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.128 0.173 0.274 0.458 0.833 1.436 2.197 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.128 0.173 0.274 0.458 0.833 1.436 2.197 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.128 0.173 0.274 0.458 0.833 1.436 2.197 O.D. 0.056 0.128 0.173 0.274 0.458 0.833 1.436 2.197

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.