Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit

Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human A2M in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced) . Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1336 1254 1452 1396 1360 61.18 4.5% Sample 2 3774 3674 3364 3354 3542 254.99 7.2% Sample 3 26412 33012 29451 29456 29583 1804.55 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1336 1254 1452 1396 1360 61.18 4.5% Sample 1 1336 1254 1452 1396 1360 61.18 4.5% Sample 2 3774 3674 3364 3354 3542 254.99 7.2% Sample 2 3774 3674 3364 3354 3542 254.99 7.2% Sample 3 26412 33012 29451 29456 29583 1804.55 6.1% Sample 3 26412 33012 29451 29456 29583 1804.55 6.1%

Gene Name

CST3

Gene ID

UniProt

P01023

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Target protein isolated from human plasma

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1336 3774 26412 1412 3693 26457 Standard deviation 93.52 233.99 1980.9 108.72 249.67 2090.1 CV (%) 7% 6.2% 7.5% 7.7% 6.3% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1336 3774 26412 1412 3693 26457 Standard deviation 93.52 233.99 1980.9 108.72 249.67 2090.1 CV (%) 7% 6.2% 7.5% 7.7% 6.3% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1336 3774 26412 1412 3693 26457 Mean (pg/ml) 1336 3774 26412 1412 3693 26457 Standard deviation 93.52 233.99 1980.9 108.72 249.67 2090.1 Standard deviation 93.52 233.99 1980.9 108.72 249.67 2090.1 CV (%) 7% 6.2% 7.5% 7.7% 6.3% 7.9% CV (%) 7% 6.2% 7.5% 7.7% 6.3% 7.9%

Sensitivity

<300 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human A2M

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Alpha-2-macroglobulin

Protein Name

Alpha-2-macroglobulin

Sample Data

Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.067 0.190 0.267 0.419 0.709 1.285 2.017 2.532 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.067 0.190 0.267 0.419 0.709 1.285 2.017 2.532 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.067 0.190 0.267 0.419 0.709 1.285 2.017 2.532 O.D. 0.067 0.190 0.267 0.419 0.709 1.285 2.017 2.532

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.