Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit

Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human ANGPTL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Angiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1210 1128 1302 1147 1197 74.2 6.2% Sample 2 4752 4862 5145 4510 4817 269.77 5.6% Sample 3 21031 21450 20486 18843 20453 859.01 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1210 1128 1302 1147 1197 74.2 6.2% Sample 1 1210 1128 1302 1147 1197 74.2 6.2% Sample 2 4752 4862 5145 4510 4817 269.77 5.6% Sample 2 4752 4862 5145 4510 4817 269.77 5.6% Sample 3 21031 21450 20486 18843 20453 859.01 4.2% Sample 3 21031 21450 20486 18843 20453 859.01 4.2%

Gene Name

CST3

Gene ID

51129

UniProt

Q9BY76

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L165-S406

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1210 4752 21031 1116 4712 21659 Standard deviation 81.07 261.36 967.43 81.47 325.13 974.66 CV (%) 6.7% 5.5% 4.6% 7.3% 6.9% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1210 4752 21031 1116 4712 21659 Standard deviation 81.07 261.36 967.43 81.47 325.13 974.66 CV (%) 6.7% 5.5% 4.6% 7.3% 6.9% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1210 4752 21031 1116 4712 21659 Mean (pg/ml) 1210 4752 21031 1116 4712 21659 Standard deviation 81.07 261.36 967.43 81.47 325.13 974.66 Standard deviation 81.07 261.36 967.43 81.47 325.13 974.66 CV (%) 6.7% 5.5% 4.6% 7.3% 6.9% 4.5% CV (%) 6.7% 5.5% 4.6% 7.3% 6.9% 4.5%

Sensitivity

<300 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human ANGPTL4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Angiopoietin-like 4

Protein Name

Angiopoietin-related protein 4

Sample Data

Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.013 0.159 0.252 0.395 0.620 0.991 1.502 2.064 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.013 0.159 0.252 0.395 0.620 0.991 1.502 2.064 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.013 0.159 0.252 0.395 0.620 0.991 1.502 2.064 O.D. 0.013 0.159 0.252 0.395 0.620 0.991 1.502 2.064

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.