Human EGF PicoKine® Quick ELISA Kit

Human EGF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human EGF in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 12 11 13 12 0.49 4.1% Sample 2 79 74 68 70 73 3.2 4.4% Sample 3 131 119 121 125 124 5.46 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 12 11 13 12 0.49 4.1% Sample 1 12 12 11 13 12 0.49 4.1% Sample 2 79 74 68 70 73 3.2 4.4% Sample 2 79 74 68 70 73 3.2 4.4% Sample 3 131 119 121 125 124 5.46 4.4% Sample 3 131 119 121 125 124 5.46 4.4%

Gene Name

CST3

Gene ID

1950

UniProt

P01133

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N971-R1023

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

1 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine

Detection Range

4.7 pg/mL - 300 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 79 131 10 84 135 Standard deviation 0.76 4.5 9.69 0.81 5.54 12.29 CV (%) 6.3% 5.7% 7.4% 8.1% 6.6% 9.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 79 131 10 84 135 Standard deviation 0.76 4.5 9.69 0.81 5.54 12.29 CV (%) 6.3% 5.7% 7.4% 8.1% 6.6% 9.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 12 79 131 10 84 135 Mean (pg/ml) 12 79 131 10 84 135 Standard deviation 0.76 4.5 9.69 0.81 5.54 12.29 Standard deviation 0.76 4.5 9.69 0.81 5.54 12.29 CV (%) 6.3% 5.7% 7.4% 8.1% 6.6% 9.1% CV (%) 6.3% 5.7% 7.4% 8.1% 6.6% 9.1%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human EGF

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Epidermal growth factor

Protein Name

Pro-epidermal growth factor

Sample Data

Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.042 0.106 0.154 0.254 0.443 0.773 1.398 2.199 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.042 0.106 0.154 0.254 0.443 0.773 1.398 2.199 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.042 0.106 0.154 0.254 0.443 0.773 1.398 2.199 O.D. 0.042 0.106 0.154 0.254 0.443 0.773 1.398 2.199

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.