Human Ficolin-1/FCN1/M Ficolin PicoKine® Quick ELISA Kit

Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1450 1365 1605 1564 1496 97.24 6.5% Sample 2 8641 7641 7712 7952 7987 263.55 3.3% Sample 3 19842 22647 21642 20458 21147 1141.95 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1450 1365 1605 1564 1496 97.24 6.5% Sample 1 1450 1365 1605 1564 1496 97.24 6.5% Sample 2 8641 7641 7712 7952 7987 263.55 3.3% Sample 2 8641 7641 7712 7952 7987 263.55 3.3% Sample 3 19842 22647 21642 20458 21147 1141.95 5.4% Sample 3 19842 22647 21642 20458 21147 1141.95 5.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1450 8641 19842 1321 8694 19651 Standard deviation 92.8 475.26 1111.15 93.79 512.95 1061.15 CV (%) 6.4% 5.5% 5.6% 7.1% 5.9% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1450 8641 19842 1321 8694 19651 Standard deviation 92.8 475.26 1111.15 93.79 512.95 1061.15 CV (%) 6.4% 5.5% 5.6% 7.1% 5.9% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1450 8641 19842 1321 8694 19651 Mean (pg/ml) 1450 8641 19842 1321 8694 19651 Standard deviation 92.8 475.26 1111.15 93.79 512.95 1061.15 Standard deviation 92.8 475.26 1111.15 93.79 512.95 1061.15 CV (%) 6.4% 5.5% 5.6% 7.1% 5.9% 5.4% CV (%) 6.4% 5.5% 5.6% 7.1% 5.9% 5.4%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.003 0.177 0.313 0.558 0.926 1.491 1.972 2.357 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.003 0.177 0.313 0.558 0.926 1.491 1.972 2.357 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.003 0.177 0.313 0.558 0.926 1.491 1.972 2.357 O.D. 0.003 0.177 0.313 0.558 0.926 1.491 1.972 2.357

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.