Human ANGPTL3 PicoKine® Quick ELISA Kit

Human ANGPTL3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human ANGPTL3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN) -like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 712 718 645 684 690 30.35 4.4% Sample 2 1841 2134 2209 1998 2046 130.91 6.4% Sample 3 9677 8512 10354 8424 9242 822.52 8.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 712 718 645 684 690 30.35 4.4% Sample 1 712 718 645 684 690 30.35 4.4% Sample 2 1841 2134 2209 1998 2046 130.91 6.4% Sample 2 1841 2134 2209 1998 2046 130.91 6.4% Sample 3 9677 8512 10354 8424 9242 822.52 8.9% Sample 3 9677 8512 10354 8424 9242 822.52 8.9%

Gene Name

CST3

Gene ID

27329

UniProt

Q9Y5C1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S17-E460

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 712 1841 9677 751 1716 9563 Standard deviation 31.33 106.78 522.56 44.31 125.27 592.91 CV (%) 4.4% 5.8% 5.4% 5.9% 7.3% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 712 1841 9677 751 1716 9563 Standard deviation 31.33 106.78 522.56 44.31 125.27 592.91 CV (%) 4.4% 5.8% 5.4% 5.9% 7.3% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 712 1841 9677 751 1716 9563 Mean (pg/ml) 712 1841 9677 751 1716 9563 Standard deviation 31.33 106.78 522.56 44.31 125.27 592.91 Standard deviation 31.33 106.78 522.56 44.31 125.27 592.91 CV (%) 4.4% 5.8% 5.4% 5.9% 7.3% 6.2% CV (%) 4.4% 5.8% 5.4% 5.9% 7.3% 6.2%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human ANGPTL3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Angiopoietin-like 3

Protein Name

Angiopoietin-related protein 3

Sample Data

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.000 0.240 0.454 0.818 1.432 2.188 2.521 2.873 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.000 0.240 0.454 0.818 1.432 2.188 2.521 2.873 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.000 0.240 0.454 0.818 1.432 2.188 2.521 2.873 O.D. 0.000 0.240 0.454 0.818 1.432 2.188 2.521 2.873

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.