Human Angiogenin/ANG PicoKine® Quick ELISA Kit

Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 120 127 102 113 6.08 5.4% Sample 2 751 691 634 721 699 42.65 6.1% Sample 3 2946 2796 2945 2864 2888 57.76 2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 120 127 102 113 6.08 5.4% Sample 1 101 120 127 102 113 6.08 5.4% Sample 2 751 691 634 721 699 42.65 6.1% Sample 2 751 691 634 721 699 42.65 6.1% Sample 3 2946 2796 2945 2864 2888 57.76 2% Sample 3 2946 2796 2945 2864 2888 57.76 2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 751 2946 98 712 2931 Standard deviation 4.44 48.06 191.49 4.41 45.57 240.34 CV (%) 4.4% 6.4% 6.5% 4.5% 6.4% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 751 2946 98 712 2931 Standard deviation 4.44 48.06 191.49 4.41 45.57 240.34 CV (%) 4.4% 6.4% 6.5% 4.5% 6.4% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 101 751 2946 98 712 2931 Mean (pg/ml) 101 751 2946 98 712 2931 Standard deviation 4.44 48.06 191.49 4.41 45.57 240.34 Standard deviation 4.44 48.06 191.49 4.41 45.57 240.34 CV (%) 4.4% 6.4% 6.5% 4.5% 6.4% 8.2% CV (%) 4.4% 6.4% 6.5% 4.5% 6.4% 8.2%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.035 0.060 0.177 0.398 0.795 1.363 1.996 2.429 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.035 0.060 0.177 0.398 0.795 1.363 1.996 2.429 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.035 0.060 0.177 0.398 0.795 1.363 1.996 2.429 O.D. 0.035 0.060 0.177 0.398 0.795 1.363 1.996 2.429

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.