Human Angiogenin/ANG PicoKine® Quick ELISA Kit

Human Angiogenin/ANG PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human ANG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 120 127 102 113 6.08 5.4% Sample 2 751 691 634 721 699 42.65 6.1% Sample 3 2946 2796 2945 2864 2888 57.76 2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 120 127 102 113 6.08 5.4% Sample 1 101 120 127 102 113 6.08 5.4% Sample 2 751 691 634 721 699 42.65 6.1% Sample 2 751 691 634 721 699 42.65 6.1% Sample 3 2946 2796 2945 2864 2888 57.76 2% Sample 3 2946 2796 2945 2864 2888 57.76 2%

Gene Name

CST3

Gene ID

283

UniProt

P03950

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q25-P147

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 751 2946 98 712 2931 Standard deviation 4.44 48.06 191.49 4.41 45.57 240.34 CV (%) 4.4% 6.4% 6.5% 4.5% 6.4% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 751 2946 98 712 2931 Standard deviation 4.44 48.06 191.49 4.41 45.57 240.34 CV (%) 4.4% 6.4% 6.5% 4.5% 6.4% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 101 751 2946 98 712 2931 Mean (pg/ml) 101 751 2946 98 712 2931 Standard deviation 4.44 48.06 191.49 4.41 45.57 240.34 Standard deviation 4.44 48.06 191.49 4.41 45.57 240.34 CV (%) 4.4% 6.4% 6.5% 4.5% 6.4% 8.2% CV (%) 4.4% 6.4% 6.5% 4.5% 6.4% 8.2%

Sensitivity

<12 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human ANG

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Angiogenin, ribonuclease, RNase A family, 5

Protein Name

Angiogenin

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.035 0.060 0.177 0.398 0.795 1.363 1.996 2.429 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.035 0.060 0.177 0.398 0.795 1.363 1.996 2.429 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.035 0.060 0.177 0.398 0.795 1.363 1.996 2.429 O.D. 0.035 0.060 0.177 0.398 0.795 1.363 1.996 2.429

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.