Rat Angiopoietin-2 PicoKine® Quick ELISA Kit

Rat Angiopoietin-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Angpt2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 265 255 217 223 240 17.52 7.3% Sample 2 1942 2098 1841 1995 1969 100.42 5.1% Sample 3 5069 4532 4756 5147 4876 238.92 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 265 255 217 223 240 17.52 7.3% Sample 1 265 255 217 223 240 17.52 7.3% Sample 2 1942 2098 1841 1995 1969 100.42 5.1% Sample 2 1942 2098 1841 1995 1969 100.42 5.1% Sample 3 5069 4532 4756 5147 4876 238.92 4.9% Sample 3 5069 4532 4756 5147 4876 238.92 4.9%

Gene Name

CST3

Gene ID

89805

UniProt

O35462

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y19-F496

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 265 1942 5069 245 1831 4768 Standard deviation 19.88 95.16 238.24 22.54 117.18 243.17 CV (%) 7.5% 4.9% 4.7% 9.2% 6.4% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 265 1942 5069 245 1831 4768 Standard deviation 19.88 95.16 238.24 22.54 117.18 243.17 CV (%) 7.5% 4.9% 4.7% 9.2% 6.4% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 265 1942 5069 245 1831 4768 Mean (pg/ml) 265 1942 5069 245 1831 4768 Standard deviation 19.88 95.16 238.24 22.54 117.18 243.17 Standard deviation 19.88 95.16 238.24 22.54 117.18 243.17 CV (%) 7.5% 4.9% 4.7% 9.2% 6.4% 5.1% CV (%) 7.5% 4.9% 4.7% 9.2% 6.4% 5.1%

Sensitivity

<15 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Rat Angpt2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Angiopoietin 2

Protein Name

Angiopoietin-2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.030 0.153 0.321 0.669 1.026 1.628 2.072 2.434 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.030 0.153 0.321 0.669 1.026 1.628 2.072 2.434 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.030 0.153 0.321 0.669 1.026 1.628 2.072 2.434 O.D. 0.030 0.153 0.321 0.669 1.026 1.628 2.072 2.434

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.