Human MMP-8 PicoKine® Quick ELISA Kit

Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 261 245 279 274 265 12.44 4.7% Sample 2 1533 1322 1368 1496 1430 78.64 5.5% Sample 3 5210 5280 4869 4875 5059 161.87 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 261 245 279 274 265 12.44 4.7% Sample 1 261 245 279 274 265 12.44 4.7% Sample 2 1533 1322 1368 1496 1430 78.64 5.5% Sample 2 1533 1322 1368 1496 1430 78.64 5.5% Sample 3 5210 5280 4869 4875 5059 161.87 3.2% Sample 3 5210 5280 4869 4875 5059 161.87 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 261 1533 5210 248 1632 5016 Standard deviation 13.83 101.18 369.91 17.11 112.61 391.25 CV (%) 5.3% 6.6% 7.1% 6.9% 6.9% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 261 1533 5210 248 1632 5016 Standard deviation 13.83 101.18 369.91 17.11 112.61 391.25 CV (%) 5.3% 6.6% 7.1% 6.9% 6.9% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 261 1533 5210 248 1632 5016 Mean (pg/ml) 261 1533 5210 248 1632 5016 Standard deviation 13.83 101.18 369.91 17.11 112.61 391.25 Standard deviation 13.83 101.18 369.91 17.11 112.61 391.25 CV (%) 5.3% 6.6% 7.1% 6.9% 6.9% 7.8% CV (%) 5.3% 6.6% 7.1% 6.9% 6.9% 7.8%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.056 0.119 0.257 0.464 0.918 1.576 1.937 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.056 0.119 0.257 0.464 0.918 1.576 1.937 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.056 0.119 0.257 0.464 0.918 1.576 1.937 O.D. 0.017 0.056 0.119 0.257 0.464 0.918 1.576 1.937

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.