Human CXCL10/IP-10 PicoKine® Quick ELISA Kit

Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10) . CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 55 61 58 60 3.54 5.9% Sample 2 245 263 269 254 258 7.22 2.8% Sample 3 894 935 842 971 911 49.17 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 55 61 58 60 3.54 5.9% Sample 1 66 55 61 58 60 3.54 5.9% Sample 2 245 263 269 254 258 7.22 2.8% Sample 2 245 263 269 254 258 7.22 2.8% Sample 3 894 935 842 971 911 49.17 5.4% Sample 3 894 935 842 971 911 49.17 5.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 245 894 69 236 874 Standard deviation 3.63 18.62 48.28 4.55 20.77 44.57 CV (%) 5.5% 7.6% 5.4% 6.6% 8.8% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 245 894 69 236 874 Standard deviation 3.63 18.62 48.28 4.55 20.77 44.57 CV (%) 5.5% 7.6% 5.4% 6.6% 8.8% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 66 245 894 69 236 874 Mean (pg/ml) 66 245 894 69 236 874 Standard deviation 3.63 18.62 48.28 4.55 20.77 44.57 Standard deviation 3.63 18.62 48.28 4.55 20.77 44.57 CV (%) 5.5% 7.6% 5.4% 6.6% 8.8% 5.1% CV (%) 5.5% 7.6% 5.4% 6.6% 8.8% 5.1%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.094 0.145 0.252 0.430 0.759 1.388 2.044 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.094 0.145 0.252 0.430 0.759 1.388 2.044 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.094 0.145 0.252 0.430 0.759 1.388 2.044 O.D. 0.025 0.094 0.145 0.252 0.430 0.759 1.388 2.044

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.