Human CXCL10/IP-10 PicoKine® Quick ELISA Kit

Human CXCL10/IP-10 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CXCL10 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10) . CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 55 61 58 60 3.54 5.9% Sample 2 245 263 269 254 258 7.22 2.8% Sample 3 894 935 842 971 911 49.17 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 55 61 58 60 3.54 5.9% Sample 1 66 55 61 58 60 3.54 5.9% Sample 2 245 263 269 254 258 7.22 2.8% Sample 2 245 263 269 254 258 7.22 2.8% Sample 3 894 935 842 971 911 49.17 5.4% Sample 3 894 935 842 971 911 49.17 5.4%

Gene Name

CST3

Gene ID

3627

UniProt

P02778

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V22-P98

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 245 894 69 236 874 Standard deviation 3.63 18.62 48.28 4.55 20.77 44.57 CV (%) 5.5% 7.6% 5.4% 6.6% 8.8% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 245 894 69 236 874 Standard deviation 3.63 18.62 48.28 4.55 20.77 44.57 CV (%) 5.5% 7.6% 5.4% 6.6% 8.8% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 66 245 894 69 236 874 Mean (pg/ml) 66 245 894 69 236 874 Standard deviation 3.63 18.62 48.28 4.55 20.77 44.57 Standard deviation 3.63 18.62 48.28 4.55 20.77 44.57 CV (%) 5.5% 7.6% 5.4% 6.6% 8.8% 5.1% CV (%) 5.5% 7.6% 5.4% 6.6% 8.8% 5.1%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CXCL10

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Chemokine (C-X-C motif) ligand 10

Protein Name

C-X-C motif chemokine 10

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.094 0.145 0.252 0.430 0.759 1.388 2.044 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.094 0.145 0.252 0.430 0.759 1.388 2.044 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.094 0.145 0.252 0.430 0.759 1.388 2.044 O.D. 0.025 0.094 0.145 0.252 0.430 0.759 1.388 2.044

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.