Human IL-1RA/IL1RN PicoKine® Quick ELISA Kit

The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 57 61 51 56 2.52 4.5% Sample 2 284 286 296 295 290 9.58 3.3% Sample 3 853 884 831 924 873 49.76 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 57 61 51 56 2.52 4.5% Sample 1 55 57 61 51 56 2.52 4.5% Sample 2 284 286 296 295 290 9.58 3.3% Sample 2 284 286 296 295 290 9.58 3.3% Sample 3 853 884 831 924 873 49.76 5.7% Sample 3 853 884 831 924 873 49.76 5.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 284 853 51 305 756 Standard deviation 4.62 20.16 46.92 3.67 24.71 49.14 CV (%) 8.4% 7.1% 5.5% 7.2% 8.1% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 284 853 51 305 756 Standard deviation 4.62 20.16 46.92 3.67 24.71 49.14 CV (%) 8.4% 7.1% 5.5% 7.2% 8.1% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 284 853 51 305 756 Mean (pg/ml) 55 284 853 51 305 756 Standard deviation 4.62 20.16 46.92 3.67 24.71 49.14 Standard deviation 4.62 20.16 46.92 3.67 24.71 49.14 CV (%) 8.4% 7.1% 5.5% 7.2% 8.1% 6.5% CV (%) 8.4% 7.1% 5.5% 7.2% 8.1% 6.5%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.033 0.124 0.149 0.253 0.428 0.760 1.413 2.023 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.033 0.124 0.149 0.253 0.428 0.760 1.413 2.023 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.033 0.124 0.149 0.253 0.428 0.760 1.413 2.023 O.D. 0.033 0.124 0.149 0.253 0.428 0.760 1.413 2.023

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.