Human IL-1RA/IL1RN PicoKine® Quick ELISA Kit

Human IL-1RA/IL1RN PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human IL1RN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 57 61 51 56 2.52 4.5% Sample 2 284 286 296 295 290 9.58 3.3% Sample 3 853 884 831 924 873 49.76 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 57 61 51 56 2.52 4.5% Sample 1 55 57 61 51 56 2.52 4.5% Sample 2 284 286 296 295 290 9.58 3.3% Sample 2 284 286 296 295 290 9.58 3.3% Sample 3 853 884 831 924 873 49.76 5.7% Sample 3 853 884 831 924 873 49.76 5.7%

Gene Name

CST3

Gene ID

3557

UniProt

P18510

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R26-E177

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 284 853 51 305 756 Standard deviation 4.62 20.16 46.92 3.67 24.71 49.14 CV (%) 8.4% 7.1% 5.5% 7.2% 8.1% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 284 853 51 305 756 Standard deviation 4.62 20.16 46.92 3.67 24.71 49.14 CV (%) 8.4% 7.1% 5.5% 7.2% 8.1% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 284 853 51 305 756 Mean (pg/ml) 55 284 853 51 305 756 Standard deviation 4.62 20.16 46.92 3.67 24.71 49.14 Standard deviation 4.62 20.16 46.92 3.67 24.71 49.14 CV (%) 8.4% 7.1% 5.5% 7.2% 8.1% 6.5% CV (%) 8.4% 7.1% 5.5% 7.2% 8.1% 6.5%

Sensitivity

<2 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human IL1RN

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 1 receptor antagonist

Protein Name

Interleukin-1 receptor antagonist protein

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.033 0.124 0.149 0.253 0.428 0.760 1.413 2.023 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.033 0.124 0.149 0.253 0.428 0.760 1.413 2.023 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.033 0.124 0.149 0.253 0.428 0.760 1.413 2.023 O.D. 0.033 0.124 0.149 0.253 0.428 0.760 1.413 2.023

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.