Human CCL7/MCP-3 PicoKine® Quick ELISA Kit

Human CCL7/MCP-3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CCL7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3) . It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 31 36 41 36 3.32 9.3% Sample 2 175 166 170 169 170 2.25 1.5% Sample 3 512 526 443 429 478 39.63 8.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 31 36 41 36 3.32 9.3% Sample 1 35 31 36 41 36 3.32 9.3% Sample 2 175 166 170 169 170 2.25 1.5% Sample 2 175 166 170 169 170 2.25 1.5% Sample 3 512 526 443 429 478 39.63 8.3% Sample 3 512 526 443 429 478 39.63 8.3%

Gene Name

CST3

Gene ID

6354

UniProt

P80098

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-L99

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 175 512 41 166 506 Standard deviation 2.77 12.78 28.16 2.83 13.11 30.87 CV (%) 7.9% 7.3% 5.5% 6.9% 7.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 175 512 41 166 506 Standard deviation 2.77 12.78 28.16 2.83 13.11 30.87 CV (%) 7.9% 7.3% 5.5% 6.9% 7.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 175 512 41 166 506 Mean (pg/ml) 35 175 512 41 166 506 Standard deviation 2.77 12.78 28.16 2.83 13.11 30.87 Standard deviation 2.77 12.78 28.16 2.83 13.11 30.87 CV (%) 7.9% 7.3% 5.5% 6.9% 7.9% 6.1% CV (%) 7.9% 7.3% 5.5% 6.9% 7.9% 6.1%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CCL7

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Chemokine (C-C motif) ligand 7

Protein Name

C-C motif chemokine 7

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.019 0.051 0.081 0.135 0.236 0.416 0.850 1.725 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.019 0.051 0.081 0.135 0.236 0.416 0.850 1.725 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.019 0.051 0.081 0.135 0.236 0.416 0.850 1.725 O.D. 0.019 0.051 0.081 0.135 0.236 0.416 0.850 1.725

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.