Human MMP-1 PicoKine® Quick ELISA Kit

Human MMP-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human MMP1 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Matrix metalloproteinase-1 (MMP-1) is also known as collagenase. Matrix metalloproteinases (MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1 (interstitial collagenase), MMP9 (gelatinase B) and MMP12 (macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 220 206 224 233 221 6.4 2.9% Sample 2 1654 1597 1541 1364 1539 78.49 5.1% Sample 3 4366 4868 4563 4965 4651 257.98 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 220 206 224 233 221 6.4 2.9% Sample 1 220 206 224 233 221 6.4 2.9% Sample 2 1654 1597 1541 1364 1539 78.49 5.1% Sample 2 1654 1597 1541 1364 1539 78.49 5.1% Sample 3 4366 4868 4563 4965 4651 257.98 5.5% Sample 3 4366 4868 4563 4965 4651 257.98 5.5%

Gene Name

CST3

Gene ID

4312

UniProt

P03956

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F20-N469

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 220 1654 4366 242 1762 4134 Standard deviation 13.86 127.36 257.59 19.6 162.1 305.92 CV (%) 6.3% 7.7% 5.9% 8.1% 9.2% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 220 1654 4366 242 1762 4134 Standard deviation 13.86 127.36 257.59 19.6 162.1 305.92 CV (%) 6.3% 7.7% 5.9% 8.1% 9.2% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 220 1654 4366 242 1762 4134 Mean (pg/ml) 220 1654 4366 242 1762 4134 Standard deviation 13.86 127.36 257.59 19.6 162.1 305.92 Standard deviation 13.86 127.36 257.59 19.6 162.1 305.92 CV (%) 6.3% 7.7% 5.9% 8.1% 9.2% 7.4% CV (%) 6.3% 7.7% 5.9% 8.1% 9.2% 7.4%

Sensitivity

<20 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human MMP1

Anticoagulant

Heparin

Gene Name Synonym

Matrix metallopeptidase 1 (interstitial collagenase)

Protein Name

Interstitial collagenase

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.200 0.330 0.579 1.062 1.824 2.345 2.441 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.200 0.330 0.579 1.062 1.824 2.345 2.441 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.200 0.330 0.579 1.062 1.824 2.345 2.441 O.D. 0.031 0.200 0.330 0.579 1.062 1.824 2.345 2.441

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.