Rat Rantes PicoKine® Quick ELISA Kit

Rat Rantes PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Ccl5 in cell culture supernatants. serum and plasma (heparin, EDTA, citrate) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 36 29 32 1.65 5.2% Sample 2 212 180 196 206 199 9.73 4.9% Sample3 364 397 405 374 385 6.93 1.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 36 29 32 1.65 5.2% Sample 1 32 30 36 29 32 1.65 5.2% Sample 2 212 180 196 206 199 9.73 4.9% Sample 2 212 180 196 206 199 9.73 4.9% Sample3 364 397 405 374 385 6.93 1.8% Sample3 364 397 405 374 385 6.93 1.8%

Gene Name

CST3

Gene ID

81780

UniProt

P50231

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-S91

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants. serum and plasma (heparin, EDTA, citrate)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 212 364 36 199 351 Standard deviation 2.02 16.32 20.02 2.74 14.13 25.62 CV (%) 6.3% 7.7% 5.5% 7.6% 7.1% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 212 364 36 199 351 Standard deviation 2.02 16.32 20.02 2.74 14.13 25.62 CV (%) 6.3% 7.7% 5.5% 7.6% 7.1% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 212 364 36 199 351 Mean (pg/ml) 32 212 364 36 199 351 Standard deviation 2.02 16.32 20.02 2.74 14.13 25.62 Standard deviation 2.02 16.32 20.02 2.74 14.13 25.62 CV (%) 6.3% 7.7% 5.5% 7.6% 7.1% 7.3% CV (%) 6.3% 7.7% 5.5% 7.6% 7.1% 7.3%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Rat Ccl5

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Chemokine (C-C motif) ligand 5

Protein Name

C-C motif chemokine 5

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.164 0.255 0.450 0.831 1.445 2.087 2.262 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.164 0.255 0.450 0.831 1.445 2.087 2.262 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.164 0.255 0.450 0.831 1.445 2.087 2.262 O.D. 0.016 0.164 0.255 0.450 0.831 1.445 2.087 2.262

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.