Human Thrombomodulin PicoKine® Quick ELISA Kit

Human Thrombomodulin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human THBD in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Thrombomodulin (TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor (TAFI, aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene (THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome (aHUS) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 166 164 170 175 169 3.71 2.2% Sample 2 420 414 399 402 409 10.22 2.5% Sample 3 1412 1301 1420 1458 1398 74.08 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 166 164 170 175 169 3.71 2.2% Sample 1 166 164 170 175 169 3.71 2.2% Sample 2 420 414 399 402 409 10.22 2.5% Sample 2 420 414 399 402 409 10.22 2.5% Sample 3 1412 1301 1420 1458 1398 74.08 5.3% Sample 3 1412 1301 1420 1458 1398 74.08 5.3%

Gene Name

CST3

Gene ID

7056

UniProt

P07204

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A19-S515

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 166 420 1412 156 466 1264 Standard deviation 7.30 26.04 87.54 9.20 34.02 92.27 CV (%) 4.4% 6.2% 6.2% 5.9% 7.3% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 166 420 1412 156 466 1264 Standard deviation 7.30 26.04 87.54 9.20 34.02 92.27 CV (%) 4.4% 6.2% 6.2% 5.9% 7.3% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 166 420 1412 156 466 1264 Mean (pg/ml) 166 420 1412 156 466 1264 Standard deviation 7.30 26.04 87.54 9.20 34.02 92.27 Standard deviation 7.30 26.04 87.54 9.20 34.02 92.27 CV (%) 4.4% 6.2% 6.2% 5.9% 7.3% 7.3% CV (%) 4.4% 6.2% 6.2% 5.9% 7.3% 7.3%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human THBD

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Thrombomodulin

Protein Name

Thrombomodulin

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.021 0.074 0.103 0.171 0.365 0.665 1.155 1.854 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.021 0.074 0.103 0.171 0.365 0.665 1.155 1.854 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.021 0.074 0.103 0.171 0.365 0.665 1.155 1.854 O.D. 0.021 0.074 0.103 0.171 0.365 0.665 1.155 1.854

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.