Human VEGF PicoKine® Quick ELISA Kit

Human VEGF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 77 81 85 82 81 4.96 6.1% Sample 2 280 266 264 251 265 11.94 4.5% Sample 3 953 890 963 998 951 52.31 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 77 81 85 82 81 4.96 6.1% Sample 1 77 81 85 82 81 4.96 6.1% Sample 2 280 266 264 251 265 11.94 4.5% Sample 2 280 266 264 251 265 11.94 4.5% Sample 3 953 890 963 998 951 52.31 5.5% Sample 3 953 890 963 998 951 52.31 5.5%

Gene Name

CST3

Gene ID

7422

UniProt

P15692

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A27-R191

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

31.2 pg/mL - 2,000 pg/mL (human serum, plasma), 15.6 pg/mL - 1,000 pg/mL (cell culture supernates)

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77 280 953 72 263 988 Standard deviation 7.32 21.84 89.58 5.54 24.72 73.11 CV (%) 9.5% 7.8% 9.4% 7.7% 9.4% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77 280 953 72 263 988 Standard deviation 7.32 21.84 89.58 5.54 24.72 73.11 CV (%) 9.5% 7.8% 9.4% 7.7% 9.4% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 77 280 953 72 263 988 Mean (pg/ml) 77 280 953 72 263 988 Standard deviation 7.32 21.84 89.58 5.54 24.72 73.11 Standard deviation 7.32 21.84 89.58 5.54 24.72 73.11 CV (%) 9.5% 7.8% 9.4% 7.7% 9.4% 7.4% CV (%) 9.5% 7.8% 9.4% 7.7% 9.4% 7.4%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human VEGFA

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Vascular endothelial growth factor A

Protein Name

Vascular endothelial growth factor A

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.130 0.338 0.523 0.912 1.480 2.228 2.473 2.536 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.130 0.338 0.523 0.912 1.480 2.228 2.473 2.536 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.130 0.338 0.523 0.912 1.480 2.228 2.473 2.536 O.D. 0.130 0.338 0.523 0.912 1.480 2.228 2.473 2.536

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.