Rat KIM1 PicoKine® Quick ELISA Kit

Rat KIM1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 93 105 102 97 5.34 5.5% Sample 2 289 271 296 302 290 16.79 5.8% Sample 3 921 881 935 899 909 34.54 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 93 105 102 97 5.34 5.5% Sample 1 88 93 105 102 97 5.34 5.5% Sample 2 289 271 296 302 290 16.79 5.8% Sample 2 289 271 296 302 290 16.79 5.8% Sample 3 921 881 935 899 909 34.54 3.8% Sample 3 921 881 935 899 909 34.54 3.8%

Gene Name

CST3

Gene ID

286934

UniProt

O54947

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S18-V238

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 289 921 84 293 985 Standard deviation 3.7 20.52 69.08 3.7 22.56 79.79 CV (%) 4.2% 7.1% 7.5% 4.4% 7.7% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 289 921 84 293 985 Standard deviation 3.7 20.52 69.08 3.7 22.56 79.79 CV (%) 4.2% 7.1% 7.5% 4.4% 7.7% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88 289 921 84 293 985 Mean (pg/ml) 88 289 921 84 293 985 Standard deviation 3.7 20.52 69.08 3.7 22.56 79.79 Standard deviation 3.7 20.52 69.08 3.7 22.56 79.79 CV (%) 4.2% 7.1% 7.5% 4.4% 7.7% 8.1% CV (%) 4.2% 7.1% 7.5% 4.4% 7.7% 8.1%

Sensitivity

<2 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Rat Havcr1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Hepatitis A virus cellular receptor 1

Protein Name

Hepatitis A virus cellular receptor 1

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.118 0.202 0.263 0.380 0.589 0.977 1.564 2.235 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.118 0.202 0.263 0.380 0.589 0.977 1.564 2.235 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.118 0.202 0.263 0.380 0.589 0.977 1.564 2.235 O.D. 0.118 0.202 0.263 0.380 0.589 0.977 1.564 2.235

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.