Human PSP94 PicoKine® Quick ELISA Kit

Human PSP94 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human MSMB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Beta-microseminoprotein, also known as MSMB or PSP94, is a protein that in humans is encoded by the MSMB gene. It is a member of the immunoglobulin binding factor family. This gene is mapped to 10q11.23. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. The expression of the encoded protein is found to be decreased in prostate cancer. But transcripts of identical size were found in prostate, respiratory (tracheal, bronchial, and lung) tissues, and the mucosa of the gastric antrum. Thus, the protein may have functions other than those related to male reproduction. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 165 155 167 152 160 3.51 2.2% Sample 2 633 650 669 736 672 32.93 4.9% Sample 3 1502 1245 1397 1305 1362 61.30 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 165 155 167 152 160 3.51 2.2% Sample 1 165 155 167 152 160 3.51 2.2% Sample 2 633 650 669 736 672 32.93 4.9% Sample 2 633 650 669 736 672 32.93 4.9% Sample 3 1502 1245 1397 1305 1362 61.30 4.5% Sample 3 1502 1245 1397 1305 1362 61.30 4.5%

Gene Name

CST3

Gene ID

4477

UniProt

P08118

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S21-I114

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 165 633 1502 143 574 1323 Standard deviation 12.87 47.48 112.65 8.72 50.51 127.01 CV (%) 7.8% 7.5% 7.5% 6.1% 8.8% 9.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 165 633 1502 143 574 1323 Standard deviation 12.87 47.48 112.65 8.72 50.51 127.01 CV (%) 7.8% 7.5% 7.5% 6.1% 8.8% 9.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 165 633 1502 143 574 1323 Mean (pg/ml) 165 633 1502 143 574 1323 Standard deviation 12.87 47.48 112.65 8.72 50.51 127.01 Standard deviation 12.87 47.48 112.65 8.72 50.51 127.01 CV (%) 7.8% 7.5% 7.5% 6.1% 8.8% 9.6% CV (%) 7.8% 7.5% 7.5% 6.1% 8.8% 9.6%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human MSMB

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Microseminoprotein beta

Protein Name

Beta-microseminoprotein

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.107 0.235 0.303 0.517 0.887 1.483 2.142 2.385 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.107 0.235 0.303 0.517 0.887 1.483 2.142 2.385 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.107 0.235 0.303 0.517 0.887 1.483 2.142 2.385 O.D. 0.107 0.235 0.303 0.517 0.887 1.483 2.142 2.385

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.