Rat EGF PicoKine® Quick ELISA Kit

Rat EGF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Egf in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 11 10 11 11 0.36 3.3% Sample 2 88 90 86 93 89 5.80 6.5% Sample 3 241 212 229 243 231 8.79 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 11 10 11 11 0.36 3.3% Sample 1 12 11 10 11 11 0.36 3.3% Sample 2 88 90 86 93 89 5.80 6.5% Sample 2 88 90 86 93 89 5.80 6.5% Sample 3 241 212 229 243 231 8.79 3.8% Sample 3 241 212 229 243 231 8.79 3.8%

Gene Name

CST3

Gene ID

25313

UniProt

P07522

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N974-R1026

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Detection Range

7.8 pg/mL - 500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 88 241 9 91 224 Standard deviation 0.79 4.66 16.87 0.73 6.28 17.47 CV (%) 6.6% 5.3% 7% 8.1% 6.9% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 88 241 9 91 224 Standard deviation 0.79 4.66 16.87 0.73 6.28 17.47 CV (%) 6.6% 5.3% 7% 8.1% 6.9% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 12 88 241 9 91 224 Mean (pg/ml) 12 88 241 9 91 224 Standard deviation 0.79 4.66 16.87 0.73 6.28 17.47 Standard deviation 0.79 4.66 16.87 0.73 6.28 17.47 CV (%) 6.6% 5.3% 7% 8.1% 6.9% 7.8% CV (%) 6.6% 5.3% 7% 8.1% 6.9% 7.8%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Rat Egf

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Epidermal growth factor

Protein Name

Pro-epidermal growth factor

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.082 0.173 0.222 0.373 0.592 1.032 1.721 2.378 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.082 0.173 0.222 0.373 0.592 1.032 1.721 2.378 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.082 0.173 0.222 0.373 0.592 1.032 1.721 2.378 O.D. 0.082 0.173 0.222 0.373 0.592 1.032 1.721 2.378

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.