Human ICAM-1/CD54 PicoKine® Quick ELISA Kit

Human ICAM-1/CD54 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human ICAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1) . ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 280 265 279 257 270 5.68 2.1% Sample 2 2022 1702 1836 1759 1830 84.17 4.6% Sample 3 4863 4823 4469 4578 4683 159.23 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 280 265 279 257 270 5.68 2.1% Sample 1 280 265 279 257 270 5.68 2.1% Sample 2 2022 1702 1836 1759 1830 84.17 4.6% Sample 2 2022 1702 1836 1759 1830 84.17 4.6% Sample 3 4863 4823 4469 4578 4683 159.23 3.4% Sample 3 4863 4823 4469 4578 4683 159.23 3.4%

Gene Name

CST3

Gene ID

3383

UniProt

P05362

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q28-E480

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 280 2022 4863 265 1960 4698 Standard deviation 14.28 113.23 248.84 15.64 129.36 248.99 CV (%) 5.1% 5.6% 4.5% 5.9% 6.6% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 280 2022 4863 265 1960 4698 Standard deviation 14.28 113.23 248.84 15.64 129.36 248.99 CV (%) 5.1% 5.6% 4.5% 5.9% 6.6% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 280 2022 4863 265 1960 4698 Mean (pg/ml) 280 2022 4863 265 1960 4698 Standard deviation 14.28 113.23 248.84 15.64 129.36 248.99 Standard deviation 14.28 113.23 248.84 15.64 129.36 248.99 CV (%) 5.1% 5.6% 4.5% 5.9% 6.6% 5.3% CV (%) 5.1% 5.6% 4.5% 5.9% 6.6% 5.3%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human ICAM1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Intercellular adhesion molecule 1

Protein Name

Intercellular adhesion molecule 1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.061 0.113 0.159 0.212 0.330 0.571 1.027 1.832 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.061 0.113 0.159 0.212 0.330 0.571 1.027 1.832 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.061 0.113 0.159 0.212 0.330 0.571 1.027 1.832 O.D. 0.061 0.113 0.159 0.212 0.330 0.571 1.027 1.832

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.