Human Laminin PicoKine® Quick ELISA Kit

Human Laminin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human Laminin in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 326 366 350 381 356 26.68 7.5% Sample 2 1022 1228 1070 1230 1138 73.94 6.5% Sample 3 4563 4223 4654 4640 4520 212.44 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 326 366 350 381 356 26.68 7.5% Sample 1 326 366 350 381 356 26.68 7.5% Sample 2 1022 1228 1070 1230 1138 73.94 6.5% Sample 2 1022 1228 1070 1230 1138 73.94 6.5% Sample 3 4563 4223 4654 4640 4520 212.44 4.7% Sample 3 4563 4223 4654 4640 4520 212.44 4.7%

Gene Name

CST3

Gene ID

284217, 3908, 3909, 3910, 3911, 3912, 3913, 3914, 22798, 3915, 3918, 10319

UniProt

P25391, P24043, Q16787, Q16363, O15230, P07942, P55268, Q13751, A4D0S4, P11047, Q13753, Q9Y6N6

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Target protein isolated from human fibroblasts

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates and serum

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 326 1022 4563 330 1120 4365 Standard deviation 23.47 49.06 337.66 29.04 62.72 410.31 CV (%) 7.2% 4.8% 7.4% 8.8% 5.6% 9.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 326 1022 4563 330 1120 4365 Standard deviation 23.47 49.06 337.66 29.04 62.72 410.31 CV (%) 7.2% 4.8% 7.4% 8.8% 5.6% 9.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 326 1022 4563 330 1120 4365 Mean (pg/ml) 326 1022 4563 330 1120 4365 Standard deviation 23.47 49.06 337.66 29.04 62.72 410.31 Standard deviation 23.47 49.06 337.66 29.04 62.72 410.31 CV (%) 7.2% 4.8% 7.4% 8.8% 5.6% 9.4% CV (%) 7.2% 4.8% 7.4% 8.8% 5.6% 9.4%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human Laminin

Gene Name Synonym

Laminin subunit alpha-1

Protein Name

Laminin subunit alpha-1; Laminin subunit beta-1; Laminin subunit gamma-1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.106 0.141 0.213 0.372 0.610 1.128 1.915 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.106 0.141 0.213 0.372 0.610 1.128 1.915 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.106 0.141 0.213 0.372 0.610 1.128 1.915 O.D. 0.056 0.106 0.141 0.213 0.372 0.610 1.128 1.915

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.