Human Elafin/PI3 PicoKine® Quick ELISA Kit

Quick ELISA Kit

Product Specifications

Background

Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 67 55 59 53 59 3.80 6.5% Sample 2 269 260 270 259 265 11.37 4.3% Sample 3 902 996 955 1033 972 56.35 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 67 55 59 53 59 3.80 6.5% Sample 1 67 55 59 53 59 3.80 6.5% Sample 2 269 260 270 259 265 11.37 4.3% Sample 2 269 260 270 259 265 11.37 4.3% Sample 3 902 996 955 1033 972 56.35 5.8% Sample 3 902 996 955 1033 972 56.35 5.8%

Gene Name

CST3

Gene ID

5266

UniProt

P19957

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A23-Q117

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 67 269 902 75 263 887 Standard deviation 4.36 12.91 50.51 5.33 14.73 60.32 CV (%) 6.5% 4.8% 5.6% 7.1% 5.6% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 67 269 902 75 263 887 Standard deviation 4.36 12.91 50.51 5.33 14.73 60.32 CV (%) 6.5% 4.8% 5.6% 7.1% 5.6% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 67 269 902 75 263 887 Mean (pg/ml) 67 269 902 75 263 887 Standard deviation 4.36 12.91 50.51 5.33 14.73 60.32 Standard deviation 4.36 12.91 50.51 5.33 14.73 60.32 CV (%) 6.5% 4.8% 5.6% 7.1% 5.6% 6.8% CV (%) 6.5% 4.8% 5.6% 7.1% 5.6% 6.8%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human PI3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Peptidase inhibitor 3, skin-derived

Protein Name

Elafin

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.154 0.244 0.461 0.821 1.425 2.122 2.274 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.154 0.244 0.461 0.821 1.425 2.122 2.274 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.154 0.244 0.461 0.821 1.425 2.122 2.274 O.D. 0.024 0.154 0.244 0.461 0.821 1.425 2.122 2.274

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.