Mouse KIM1/TIM1 PicoKine® Quick ELISA Kit

KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 91 91 99 91 3.19 3.5% Sample 2 296 269 241 277 271 13.81 5.1% Sample 3 863 722 826 797 802 42.51 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 91 91 99 91 3.19 3.5% Sample 1 83 91 91 99 91 3.19 3.5% Sample 2 296 269 241 277 271 13.81 5.1% Sample 2 296 269 241 277 271 13.81 5.1% Sample 3 863 722 826 797 802 42.51 5.3% Sample 3 863 722 826 797 802 42.51 5.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 296 863 79 265 805 Standard deviation 6.47 16.28 36.25 6.95 14.05 37.84 CV (%) 7.8% 5.5% 4.2% 8.8% 5.3% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 296 863 79 265 805 Standard deviation 6.47 16.28 36.25 6.95 14.05 37.84 CV (%) 7.8% 5.5% 4.2% 8.8% 5.3% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 83 296 863 79 265 805 Mean (pg/ml) 83 296 863 79 265 805 Standard deviation 6.47 16.28 36.25 6.95 14.05 37.84 Standard deviation 6.47 16.28 36.25 6.95 14.05 37.84 CV (%) 7.8% 5.5% 4.2% 8.8% 5.3% 4.7% CV (%) 7.8% 5.5% 4.2% 8.8% 5.3% 4.7%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.