Human MBL2/MPB-C PicoKine® Quick ELISA Kit

Human MBL2/MPB-C PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human MBL2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 482 544 480 477 496 31.23 6.3% Sample 2 2754 2813 2929 2845 2835 59.54 2.1% Sample 3 8841 9907 8436 9421 9151 503.32 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 482 544 480 477 496 31.23 6.3% Sample 1 482 544 480 477 496 31.23 6.3% Sample 2 2754 2813 2929 2845 2835 59.54 2.1% Sample 2 2754 2813 2929 2845 2835 59.54 2.1% Sample 3 8841 9907 8436 9421 9151 503.32 5.5% Sample 3 8841 9907 8436 9421 9151 503.32 5.5%

Gene Name

CST3

Gene ID

4153

UniProt

P11226

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-I248

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 482 2754 8841 446 2879 8912 Standard deviation 26.99 126.68 680.76 33 149.71 730.78 CV (%) 5.6% 4.6% 7.7% 7.4% 5.2% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 482 2754 8841 446 2879 8912 Standard deviation 26.99 126.68 680.76 33 149.71 730.78 CV (%) 5.6% 4.6% 7.7% 7.4% 5.2% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 482 2754 8841 446 2879 8912 Mean (pg/ml) 482 2754 8841 446 2879 8912 Standard deviation 26.99 126.68 680.76 33 149.71 730.78 Standard deviation 26.99 126.68 680.76 33 149.71 730.78 CV (%) 5.6% 4.6% 7.7% 7.4% 5.2% 8.2% CV (%) 5.6% 4.6% 7.7% 7.4% 5.2% 8.2%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human MBL2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Mannose-binding lectin (protein C) 2, soluble

Protein Name

Mannose-binding protein C

Sample Data

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.027 0.388 0.693 1.231 2.064 2.644 2.417 2.577 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.027 0.388 0.693 1.231 2.064 2.644 2.417 2.577 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.027 0.388 0.693 1.231 2.064 2.644 2.417 2.577 O.D. 0.027 0.388 0.693 1.231 2.064 2.644 2.417 2.577

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.