Human Kallistatin/Serpina4 PicoKine® Quick ELISA Kit

Human Kallistatin/Serpina4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human SERPINA4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 251 266 244 301 266 16.20 6.1% Sample 2 1712 1764 1650 1844 1743 50.53 2.9% Sample 3 4369 4597 4696 4723 4596 133.29 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 251 266 244 301 266 16.20 6.1% Sample 1 251 266 244 301 266 16.20 6.1% Sample 2 1712 1764 1650 1844 1743 50.53 2.9% Sample 2 1712 1764 1650 1844 1743 50.53 2.9% Sample 3 4369 4597 4696 4723 4596 133.29 2.9% Sample 3 4369 4597 4696 4723 4596 133.29 2.9%

Gene Name

CST3

Gene ID

5267

UniProt

P29622

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-P427

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 251 1712 4269 229 1563 4117 Standard deviation 12.05/td> 94.16 204.91 12.37 90.65 251.14 CV (%) 4.8% 5.5% 4.8% 5.4% 5.8% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 251 1712 4269 229 1563 4117 Standard deviation 12.05/td> 94.16 204.91 12.37 90.65 251.14 CV (%) 4.8% 5.5% 4.8% 5.4% 5.8% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 251 1712 4269 229 1563 4117 Mean (pg/ml) 251 1712 4269 229 1563 4117 Standard deviation 12.05/td> 94.16 204.91 12.37 90.65 251.14 Standard deviation 12.05/td> 94.16 204.91 12.37 90.65 251.14 CV (%) 4.8% 5.5% 4.8% 5.4% 5.8% 6.1% CV (%) 4.8% 5.5% 4.8% 5.4% 5.8% 6.1%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human SERPINA4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4

Protein Name

Kallistatin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.010 0.132 0.225 0.431 0.753 1.438 2.208 2.404 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.010 0.132 0.225 0.431 0.753 1.438 2.208 2.404 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.010 0.132 0.225 0.431 0.753 1.438 2.208 2.404 O.D. 0.010 0.132 0.225 0.431 0.753 1.438 2.208 2.404

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.