Rat MMP-2 PicoKine® Quick ELISA Kit

Rat MMP-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2) . It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch) . The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2001 1093 986 1110 1298 63.58 4.9% Sample 2 4516 3756 4531 4695 4375 253.72 5.8% Sample 3 23614 23697 22457 26546 24079 1396.55 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2001 1093 986 1110 1298 63.58 4.9% Sample 1 2001 1093 986 1110 1298 63.58 4.9% Sample 2 4516 3756 4531 4695 4375 253.72 5.8% Sample 2 4516 3756 4531 4695 4375 253.72 5.8% Sample 3 23614 23697 22457 26546 24079 1396.55 5.8% Sample 3 23614 23697 22457 26546 24079 1396.55 5.8%

Gene Name

CST3

Gene ID

81686

UniProt

P33436

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A30-C662

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin)

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2001 4516 23614 1093 4543 21263 Standard deviation 176.09 320.64 1157.09 86.35 367.98 1318.31 CV (%) 8.8% 7.1% 4.9% 7.9% 8.1% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2001 4516 23614 1093 4543 21263 Standard deviation 176.09 320.64 1157.09 86.35 367.98 1318.31 CV (%) 8.8% 7.1% 4.9% 7.9% 8.1% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2001 4516 23614 1093 4543 21263 Mean (pg/ml) 2001 4516 23614 1093 4543 21263 Standard deviation 176.09 320.64 1157.09 86.35 367.98 1318.31 Standard deviation 176.09 320.64 1157.09 86.35 367.98 1318.31 CV (%) 8.8% 7.1% 4.9% 7.9% 8.1% 6.2% CV (%) 8.8% 7.1% 4.9% 7.9% 8.1% 6.2%

Sensitivity

<300 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Rat Mmp2

Anticoagulant

Heparin

Gene Name Synonym

Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

Protein Name

72 kDa type IV collagenase

Sample Data

Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.029 0.115 0.173 0.310 0.537 0.976 1.555 2.153 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.029 0.115 0.173 0.310 0.537 0.976 1.555 2.153 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.029 0.115 0.173 0.310 0.537 0.976 1.555 2.153 O.D. 0.029 0.115 0.173 0.310 0.537 0.976 1.555 2.153

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.