Human SPINT1/HAI-1 PicoKine® Quick ELISA Kit

Human SPINT1/HAI-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human SPINT1 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 211 244 208 233 224 12.32 5.5% Sample 2 1855 1675 1694 1706 1733 39.85 2.3% Sample 3 3887 4423 4263 4699 4318 250.44 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 211 244 208 233 224 12.32 5.5% Sample 1 211 244 208 233 224 12.32 5.5% Sample 2 1855 1675 1694 1706 1733 39.85 2.3% Sample 2 1855 1675 1694 1706 1733 39.85 2.3% Sample 3 3887 4423 4263 4699 4318 250.44 5.8% Sample 3 3887 4423 4263 4699 4318 250.44 5.8%

Gene Name

CST3

Gene ID

6692

UniProt

O43278

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G36-L529

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 211 1855 3887 234 1852 3784 Standard deviation 13.5 116.87 264.32 17.55 120.38 298.94 CV (%) 6.4% 6.3% 6.8% 7.5% 6.5% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 211 1855 3887 234 1852 3784 Standard deviation 13.5 116.87 264.32 17.55 120.38 298.94 CV (%) 6.4% 6.3% 6.8% 7.5% 6.5% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 211 1855 3887 234 1852 3784 Mean (pg/ml) 211 1855 3887 234 1852 3784 Standard deviation 13.5 116.87 264.32 17.55 120.38 298.94 Standard deviation 13.5 116.87 264.32 17.55 120.38 298.94 CV (%) 6.4% 6.3% 6.8% 7.5% 6.5% 7.9% CV (%) 6.4% 6.3% 6.8% 7.5% 6.5% 7.9%

Sensitivity

<15 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human SPINT1

Anticoagulant

Heparin

Gene Name Synonym

Serine peptidase inhibitor, Kunitz type 1

Protein Name

Kunitz-type protease inhibitor 1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.102 0.150 0.243 0.421 0.765 1.383 2.228 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.102 0.150 0.243 0.421 0.765 1.383 2.228 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.102 0.150 0.243 0.421 0.765 1.383 2.228 O.D. 0.036 0.102 0.150 0.243 0.421 0.765 1.383 2.228

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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