Human Cathepsin D PicoKine® Quick ELISA Kit

Human Cathepsin D PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CTSD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 191 201 226 217 209 11.48 5.5% Sample 2 1701 1814 1695 1596 1702 76.57 4.5% Sample 3 5274 5267 4806 4977 5081 157.51 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 191 201 226 217 209 11.48 5.5% Sample 1 191 201 226 217 209 11.48 5.5% Sample 2 1701 1814 1695 1596 1702 76.57 4.5% Sample 2 1701 1814 1695 1596 1702 76.57 4.5% Sample 3 5274 5267 4806 4977 5081 157.51 3.1% Sample 3 5274 5267 4806 4977 5081 157.51 3.1%

Gene Name

CST3

Gene ID

1509

UniProt

P07339

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-L412

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 191 1701 5274 179 1699 5098 Standard deviation 13.56 100.36 358.63 13.43 108.74 433.33 CV (%) 7.1% 5.9% 6.8% 7.5% 6.4% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 191 1701 5274 179 1699 5098 Standard deviation 13.56 100.36 358.63 13.43 108.74 433.33 CV (%) 7.1% 5.9% 6.8% 7.5% 6.4% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 191 1701 5274 179 1699 5098 Mean (pg/ml) 191 1701 5274 179 1699 5098 Standard deviation 13.56 100.36 358.63 13.43 108.74 433.33 Standard deviation 13.56 100.36 358.63 13.43 108.74 433.33 CV (%) 7.1% 5.9% 6.8% 7.5% 6.4% 8.5% CV (%) 7.1% 5.9% 6.8% 7.5% 6.4% 8.5%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CTSD

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cathepsin D

Protein Name

Cathepsin D

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.