Human Azurocidin PicoKine® Quick ELISA Kit

Human Azurocidin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human AZU1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Azurocidin, also known as cationic antimicrobial protein CAP37 or heparin-binding protein (HBP), is a protein that in humans is encoded by the AZU1 gene. This encoded protein is a member of the serine protease gene family, but it is not a serine proteinase, because the active site serine and histidine residues are replaced. Azurocidin is mapped to 19p13.3. The protein encoded by this gene is an azurophil granule antibiotic protein, with antibacterial activity. It is also an important multifunctional inflammatory mediator. In addition to it, Azurocidin is also a specific chemoattractant for monocytes. It lacks the chemotactic activity for neutrophils and lymphocytes, and this gene is probably responsible for the wave of monocytes that follows the initial wave of PMNs typical of the inflammatory response.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 191 180 187 202 190 10.83 5.7% Sample 2 617 589 606 634 612 40.97 6.7% Sample 3 1152 1198 1201 1057 1152 48.38 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 191 180 187 202 190 10.83 5.7% Sample 1 191 180 187 202 190 10.83 5.7% Sample 2 617 589 606 634 612 40.97 6.7% Sample 2 617 589 606 634 612 40.97 6.7% Sample 3 1152 1198 1201 1057 1152 48.38 4.2% Sample 3 1152 1198 1201 1057 1152 48.38 4.2%

Gene Name

CST3

Gene ID

566

UniProt

P20160

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I27-P248

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 191 617 1152 185 604 1089 Standard deviation 10.12 38.87 81.79 9.99 39.86 95.83 CV (%) 5.3% 6.3% 7.1% 5.4% 6.6% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 191 617 1152 185 604 1089 Standard deviation 10.12 38.87 81.79 9.99 39.86 95.83 CV (%) 5.3% 6.3% 7.1% 5.4% 6.6% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 191 617 1152 185 604 1089 Mean (pg/ml) 191 617 1152 185 604 1089 Standard deviation 10.12 38.87 81.79 9.99 39.86 95.83 Standard deviation 10.12 38.87 81.79 9.99 39.86 95.83 CV (%) 5.3% 6.3% 7.1% 5.4% 6.6% 8.8% CV (%) 5.3% 6.3% 7.1% 5.4% 6.6% 8.8%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human AZU1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Azurocidin 1

Protein Name

Azurocidin

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.059 0.138 0.202 0.332 0.560 0.969 1.857 2.321 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.059 0.138 0.202 0.332 0.560 0.969 1.857 2.321 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.059 0.138 0.202 0.332 0.560 0.969 1.857 2.321 O.D. 0.059 0.138 0.202 0.332 0.560 0.969 1.857 2.321

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.