Human CD200 PicoKine® Quick ELISA Kit

Human CD200 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CD200 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 75 93 98 88 4.66 5.3% Sample 2 252 245 216 254 241 7.47 3.1% Sample 3 897 862 897 871 881 22.02 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 75 93 98 88 4.66 5.3% Sample 1 89 75 93 98 88 4.66 5.3% Sample 2 252 245 216 254 241 7.47 3.1% Sample 2 252 245 216 254 241 7.47 3.1% Sample 3 897 862 897 871 881 22.02 2.5% Sample 3 897 862 897 871 881 22.02 2.5%

Gene Name

CST3

Gene ID

4345

UniProt

P41217

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q31-G232

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 252 897 75 251 9606 Standard deviation 4.36 16.12 69.06 4.12 14.30 72 CV (%) 4.9% 6.4% 7.7% 5.5% 5.7% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 252 897 75 251 9606 Standard deviation 4.36 16.12 69.06 4.12 14.30 72 CV (%) 4.9% 6.4% 7.7% 5.5% 5.7% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 89 252 897 75 251 9606 Mean (pg/ml) 89 252 897 75 251 9606 Standard deviation 4.36 16.12 69.06 4.12 14.30 72 Standard deviation 4.36 16.12 69.06 4.12 14.30 72 CV (%) 4.9% 6.4% 7.7% 5.5% 5.7% 7.5% CV (%) 4.9% 6.4% 7.7% 5.5% 5.7% 7.5%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CD200

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.062 0.099 0.185 0.329 0.625 1.179 2.046 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.062 0.099 0.185 0.329 0.625 1.179 2.046 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.062 0.099 0.185 0.329 0.625 1.179 2.046 O.D. 0.015 0.062 0.099 0.185 0.329 0.625 1.179 2.046

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.