Human IL-4 PicoKine® Quick ELISA Kit

Human IL-4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human IL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 42 35 40 33 37 1.59 4.3% Sample 2 224 195 213 207 209 8.77 4.2% Sample 3 543 445 515 487 497 27.33 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 42 35 40 33 37 1.59 4.3% Sample 1 42 35 40 33 37 1.59 4.3% Sample 2 224 195 213 207 209 8.77 4.2% Sample 2 224 195 213 207 209 8.77 4.2% Sample 3 543 445 515 487 497 27.33 5.5% Sample 3 543 445 515 487 497 27.33 5.5%

Gene Name

IL4

Gene ID

3565

UniProt

P05112

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H25-S153

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 224 543 33 199 537 Standard deviation 2.26 12.09 25.52 2.14 10.94 29.53 CV (%) 5.4% 5.4% 4.7% 6.5% 6.6% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 224 543 33 199 537 Standard deviation 2.26 12.09 25.52 2.14 10.94 29.53 CV (%) 5.4% 5.4% 4.7% 6.5% 6.6% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 42 224 543 33 199 537 Mean (pg/ml) 42 224 543 33 199 537 Standard deviation 2.26 12.09 25.52 2.14 10.94 29.53 Standard deviation 2.26 12.09 25.52 2.14 10.94 29.53 CV (%) 5.4% 5.4% 4.7% 6.5% 6.6% 5.5% CV (%) 5.4% 5.4% 4.7% 6.5% 6.6% 5.5%

Sensitivity

<1.5 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human IL4

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.040 0.148 0.238 0.416 0.755 1.314 2.094 2.570 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.040 0.148 0.238 0.416 0.755 1.314 2.094 2.570 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.040 0.148 0.238 0.416 0.755 1.314 2.094 2.570 O.D. 0.040 0.148 0.238 0.416 0.755 1.314 2.094 2.570

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.